4EB1

Hyperstable in-frame insertion variant of antithrombin

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (I)	Antithrombin-III	polymer	440	49927.0	1	UniProt (P01008) Pfam (PF00079)	Homo sapiens (human)	ATIII, Serpin C1
2	B (L)	Antithrombin-III	polymer	432	49101.0	1	UniProt (P01008) Pfam (PF00079)	Homo sapiens (human)	latent alpha antithrombin, ATIII, Serpin C1
3	C, D (A, B)	2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose	branched		424.4	2	In PDB GlyTouCan (G42666HT)
4	E, F (I)	2-acetamido-2-deoxy-beta-D-glucopyranose	non-polymer		221.2	2	Chemie (NAG)
5	G, H (I, L)	water	water		18.0	27	Chemie (HOH)

Sequence modifications

I: 1 - 208 (UniProt: P01008)

PDB	External Database	Details
Ala 137	Ser 169	engineered mutation

I: 219 - 440 (UniProt: P01008)

PDB	External Database	Details
Val 209	-	insertion
Leu 210	-	insertion
Val 211	-	insertion
Leu 212	-	insertion
Val 213	-	insertion
Asn 214	-	insertion
Thr 215	-	insertion
Arg 216	-	insertion
Thr 217	-	insertion
Ser 218	-	insertion

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	99028.0
Branched	Number of molecules	2
	Total formula weight	848.8
Non-Polymers*	Number of molecules	2
	Total formula weight	442.4
All*	Total formula weight	100319.3

*Water molecules are not included.