4EAL

Co-crystal of AMPK core with ATP soaked with AMP

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	5'-AMP-activated protein kinase catalytic subunit alpha-1	polymer	106	12097.9	1	UniProt (P54645) Pfam (PF16579) In PDB	Rattus norvegicus (Rat)	AMPK subunit alpha-1,Acetyl-CoA carboxylase kinase,ACACA kinase,Hydroxymethylglutaryl-CoA reductase kinase,HMGCR kinase,Tau-protein kinase PRKAA1
2	B	5'-AMP-activated protein kinase subunit beta-1	polymer	72	8133.8	1	UniProt (P80386) Pfam (PF04739) In PDB	Rattus norvegicus (Rat)	AMPKb,5'-AMP-activated protein kinase 40 kDa subunit
3	C	5'-AMP-activated protein kinase subunit gamma-1	polymer	330	37434.1	1	UniProt (P80385) Pfam (PF00571) In PDB	Rattus norvegicus (Rat)	AMPKg
4	C	ADENOSINE MONOPHOSPHATE	non-polymer		347.2	2	Chemie (AMP)
5		water	water		18.0	58	Chemie (HOH)

Sequence modifications

A: 394 - 522 (UniProt: P54645)

PDB	External Database	Details
Gly 389	-	expression tag
Pro 390	-	expression tag
His 391	-	expression tag
Met 392	-	expression tag
Gly 393	-	expression tag

A: 529 - 548 (UniProt: P54645)

PDB	External Database	Details
Gly 523	-	linker
Gly 524	-	linker
Gly 525	-	linker
Gly 526	-	linker
Gly 527	-	linker
Gly 528	-	linker

B: 200 - 270 (UniProt: P80386)

PDB	External Database	Details
Met 199	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	3
	Total formula weight	57665.8
Non-Polymers*	Number of molecules	2
	Total formula weight	694.4
All*	Total formula weight	58360.2

*Water molecules are not included.