4E97

T4 Lysozyme L99A/M102H with 2-Mercaptoethanol Bound

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (A, B)	Lysozyme	polymer	187	21404.4	2	UniProt (P00720) Pfam (PF00959)	Enterobacteria phage T4	Endolysin, Lysis protein, Muramidase
2	C, D, H, I, J... (A, B)	BETA-MERCAPTOETHANOL	non-polymer		78.1	8	Chemie (BME)
3	E, F, G, O, P (A, B)	SULFATE ION	non-polymer		96.1	5	Chemie (SO4)
4	K (A)	2-HYDROXYETHYL DISULFIDE	non-polymer		154.3	1	Chemie (HED)
5	L, Q (B)	ACETATE ION	non-polymer		59.0	2	Chemie (ACT)
6	S, T (A, B)	water	water		18.0	413	Chemie (HOH)

Sequence modifications

A, B: 1 - 164 (UniProt: P00720)

PDB	External Database	Details
Met -22	-	expression tag
Gly -21	-	expression tag
His -20	-	expression tag
His -19	-	expression tag
His -18	-	expression tag
His -17	-	expression tag
His -16	-	expression tag
His -15	-	expression tag
Asp -14	-	expression tag
Tyr -13	-	expression tag
Asp -12	-	expression tag
Ile -11	-	expression tag
Pro -10	-	expression tag
Thr -9	-	expression tag
Thr -8	-	expression tag
Glu -7	-	expression tag
Asn -6	-	expression tag
Leu -5	-	expression tag
Tyr -4	-	expression tag
Phe -3	-	expression tag
Gln -2	-	expression tag
Gly -1	-	expression tag
Ser 0	-	expression tag
Cys 21	Thr 21	engineered mutation
Asp 38	Ser 38	engineered mutation
Ala 99	Leu 99	engineered mutation
His 102	Met 102	engineered mutation
Val 108	Glu 108	engineered mutation
Val 117	Ser 117	engineered mutation
Cys 142	Thr 142	engineered mutation
Asp 144	Asn 144	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	42808.9
Non-Polymers*	Number of molecules	16
	Total formula weight	1377.7
All*	Total formula weight	44186.6

*Water molecules are not included.