4E7A

Crystal structure of a product state assembly of HCV NS5B genotype 2a JFH-1 isolate with beta hairpin deletion bound to primer-template RNA with a 2',3'-ddC

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (T, P)	5'-R(*CP*AP*UP*GP*GP*C)-D(P*(DOC))-3'	polymer	7	2164.4	2
2	C (A)	RNA-directed RNA polymerase	polymer	572	63736.2	1	UniProt (Q99IB8) Pfam (PF00998)	Hepatitis C virus (HCV)	NS5B, p68
3	D, E (P, A)	water	water		18.0	18	Chemie (HOH)

Sequence modifications

A: 1 - 443 (UniProt: Q99IB8)

PDB	External Database	Details
Met -1	-	expression tag
Ser 0	-	expression tag
Gln 86	Glu 2528	engineered mutation
Gln 87	Glu 2529	engineered mutation

A: 454 - 570 (UniProt: Q99IB8)

PDB	External Database	Details
Gly 444	-	linker
Gly 445	-	linker
Leu 571	-	expression tag
Glu 572	-	expression tag
His 573	-	expression tag
His 574	-	expression tag
His 575	-	expression tag
His 576	-	expression tag
His 577	-	expression tag
His 578	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	3
	Total formula weight	68064.9
All*	Total formula weight	68064.9

*Water molecules are not included.