4E76

Apo crystal structure of HCV NS5B genotype 2A JFH-1 isolate with beta hairpin loop deletion

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	RNA-directed RNA polymerase	polymer	572	63736.2	1	UniProt (Q99IB8) Pfam (PF00998)	Hepatitis C virus (HCV)	NS5B, p68
2	B, C, D, E, F... (A)	SULFATE ION	non-polymer		96.1	7	Chemie (SO4)
3	I (A)	1,2-ETHANEDIOL	non-polymer		62.1	1	Chemie (EDO)
4	J (A)	water	water		18.0	169	Chemie (HOH)

Sequence modifications

A: 1 - 443 (UniProt: Q99IB8)

PDB	External Database	Details
Met -1	-	expression tag
Ser 0	-	expression tag
Gln 86	Glu 2528	engineered mutation
Gln 87	Glu 2529	engineered mutation

A: 454 - 570 (UniProt: Q99IB8)

PDB	External Database	Details
Gly 444	-	linker
Gly 445	-	linker
Leu 571	-	expression tag
Glu 572	-	expression tag
His 573	-	expression tag
His 574	-	expression tag
His 575	-	expression tag
His 576	-	expression tag
His 577	-	expression tag
His 578	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	1
	Total formula weight	63736.2
Non-Polymers*	Number of molecules	8
	Total formula weight	734.5
All*	Total formula weight	64470.7

*Water molecules are not included.