4E56
Crystal Structure of spacer 8aa-shortened cephalosporin acylase mutant
Entity
Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
1 | A, B | Cephalosporin acylase | polymer | 696 | 77447.1 | 2 | UniProt (O86089) Pfam (PF01804) In PDB | Pseudomonas sp. | |
2 | A, B | CALCIUM ION | non-polymer | 40.1 | 2 | Chemie (CA) | |||
3 | water | water | 18.0 | 1236 | Chemie (HOH) |
Sequence modifications
A, B: 4 - 684 (UniProt: O86089)
PDB | External Database | Details |
---|---|---|
Met 1 | - | expression tag |
Gly 2 | - | expression tag |
Ile 3 | - | expression tag |
Glu 127 | Asp 154 | SEE REMARK 999 |
- | Asp 190 | deletion |
- | Pro 191 | deletion |
- | Pro 192 | deletion |
- | Asp 193 | deletion |
- | Leu 194 | deletion |
- | Ala 195 | deletion |
- | Asp 196 | deletion |
- | Gln 197 | deletion |
Ala 164 | Ser 199 | engineered mutation |
Leu 685 | - | expression tag |
Ala 686 | - | expression tag |
Ala 687 | - | expression tag |
Ala 688 | - | expression tag |
Leu 689 | - | expression tag |
Glu 690 | - | expression tag |
His 691 | - | expression tag |
His 692 | - | expression tag |
His 693 | - | expression tag |
His 694 | - | expression tag |
His 695 | - | expression tag |
His 696 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
Polymers | Number of chains | 2 |
Total formula weight | 154894.2 | |
Non-Polymers* | Number of molecules | 2 |
Total formula weight | 80.2 | |
All* | Total formula weight | 154974.3 |