4E55

Crystal Structure of spacer removed cephalosporin acylase mutant

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (A, B)	Cephalosporin acylase	polymer	694	77333.0	2	UniProt (O86089) Pfam (PF01804)	Pseudomonas sp.
2	C, D (A, B)	CALCIUM ION	non-polymer		40.1	2	Chemie (CA)
3	E, F (A, B)	water	water		18.0	915	Chemie (HOH)

Sequence modifications

A, B: 4 - 682 (UniProt: O86089)

PDB	External Database	Details
Met 1	-	expression tag
Gly 2	-	expression tag
Ile 3	-	expression tag
Glu 127	Asp 154	SEE REMARK 999
-	Gly 189	deletion
-	Asp 190	deletion
-	Pro 191	deletion
-	Pro 192	deletion
-	Asp 193	deletion
-	Leu 194	deletion
-	Ala 195	deletion
-	Asp 196	deletion
-	Gln 197	deletion
-	Gly 198	deletion
Ala 162	Ser 199	engineered mutation
Leu 683	-	expression tag
Ala 684	-	expression tag
Ala 685	-	expression tag
Ala 686	-	expression tag
Leu 687	-	expression tag
Glu 688	-	expression tag
His 689	-	expression tag
His 690	-	expression tag
His 691	-	expression tag
His 692	-	expression tag
His 693	-	expression tag
His 694	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	154666.0
Non-Polymers*	Number of molecules	2
	Total formula weight	80.2
All*	Total formula weight	154746.1

*Water molecules are not included.