4E3R

PLP-bound aminotransferase mutant crystal structure from Vibrio fluvialis

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C, D (A, B, C, D)	Pyruvate transaminase	polymer	473	52448.3	4	UniProt (F2XBU9) Pfam (PF00202)	Vibrio fluvialis	aminotransferase
2	E, G, J (A, B, D)	SULFATE ION	non-polymer		96.1	3	Chemie (SO4)
3	F, H, I, K (A, B, C, D)	SODIUM ION	non-polymer		23.0	4	Chemie (NA)
4	L, M, N, O (A, B, C, D)	water	water		18.0	1497	Chemie (HOH)

Sequence modifications

A, B, C, D: 1 - 453 (UniProt: F2XBU9)

PDB	External Database	Details
Met -19	-	expression tag
Gly -18	-	expression tag
Ser -17	-	expression tag
Ser -16	-	expression tag
His -15	-	expression tag
His -14	-	expression tag
His -13	-	expression tag
His -12	-	expression tag
His -11	-	expression tag
His -10	-	expression tag
Ser -9	-	expression tag
Ser -8	-	expression tag
Gly -7	-	expression tag
Leu -6	-	expression tag
Val -5	-	expression tag
Pro -4	-	expression tag
Arg -3	-	expression tag
Gly -2	-	expression tag
Ser -1	-	expression tag
His 0	-	expression tag
Trp 19	Phe 19	engineered mutation
Phe 57	Trp 57	engineered mutation
Ala 85	Phe 85	engineered mutation
Lys 88	Arg 88	engineered mutation
Ala 153	Val 153	engineered mutation
Phe 163	Lys 163	engineered mutation
Val 259	Ile 259	engineered mutation
Phe 415	Arg 415	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	209793.3
Non-Polymers*	Number of molecules	7
	Total formula weight	380.1
All*	Total formula weight	210173.4

*Water molecules are not included.