4E2X
X-ray Structure of the Y222F mutant of TcaB9, a C-3'-Methyltransferase, in Complex with S-Adenosyl-L-Homocysteine and dTDP
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (A) | TcaB9 | polymer | 416 | 46074.9 | 1 | UniProt (B5L6K6) Pfam (PF08421) Pfam (PF13489) Pfam (PF08484) | Micromonospora chalcea | C-3'-Methyltransferase |
| 2 | B (A) | ZINC ION | non-polymer | 65.4 | 1 | Chemie (ZN) | |||
| 3 | C, D, E, F (A) | 1,2-ETHANEDIOL | non-polymer | 62.1 | 4 | Chemie (EDO) | |||
| 4 | G (A) | S-ADENOSYL-L-HOMOCYSTEINE | non-polymer | 384.4 | 1 | Chemie (SAH) | |||
| 5 | H (A) | THYMIDINE-5'-DIPHOSPHATE | non-polymer | 402.2 | 1 | Chemie (TYD) | |||
| 6 | I (A) | water | water | 18.0 | 473 | Chemie (HOH) |
Sequence modifications
A: 1 - 414 (UniProt: B5L6K6)
| PDB | External Database | Details |
|---|---|---|
| Gly -1 | - | expression tag |
| His 0 | - | expression tag |
| Phe 222 | Tyr 222 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 1 |
| Total formula weight | 46074.9 | |
| Non-Polymers* | Number of molecules | 7 |
| Total formula weight | 1100.3 | |
| All* | Total formula weight | 47175.2 |
