4E2W
X-ray Structure of the H181N mutant of TcaB9, a C-3'-Methyltransferase, in Complex with S-Adenosyl-L-Homocysteine and Sugar Product
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (A) | TcaB9 | polymer | 416 | 46066.9 | 1 | UniProt (B5L6K6) | Micromonospora chalcea | C-3'-Methyltransferase |
| 2 | B (A) | ZINC ION | non-polymer | 65.4 | 1 | Chemie (ZN) | |||
| 3 | C, D, E (A) | 1,2-ETHANEDIOL | non-polymer | 62.1 | 3 | Chemie (EDO) | |||
| 4 | F (A) | S-ADENOSYL-L-HOMOCYSTEINE | non-polymer | 384.4 | 1 | Chemie (SAH) PubChem (16760024) PubChem (193) PubChem (13792) PubChem (177678424) PubChem (189149) PubChem (439155) PubChem (25210495) PubChem (21125603) PubChem (44361184) PubChem (25203782) PubChem (46936206) PubChem (50989525) PubChem (51380914) PubChem (52224398) PubChem (57544532) PubChem (52224400) PubChem (59046843) PubChem (59170628) PubChem (59576228) PubChem (66805708) PubChem (5284331) PubChem (70689219) PubChem (70702054) PubChem (70832431) PubChem (71170554) PubChem (13231890) PubChem (6419189) PubChem (118374891) PubChem (118560068) PubChem (126969526) PubChem (129105892) PubChem (164157219) PubChem (143518505) PubChem (154578335) PubChem (163054214) PubChem (163335525) | |||
| 5 | G (A) | (2R,4S,6R)-4-amino-4,6-dimethyl-5-oxotetrahydro-2H-pyran-2-yl [(2R,3S,5R)-3-hydroxy-5-(5-methyl-2,4-dioxo-3,4-dihydropyrimidin-1(2H)-yl)tetrahydrofuran-2-yl]methyl dihydrogen diphosphate (non-preferred name) | non-polymer | 543.4 | 1 | Chemie (JHZ) PubChem (24892776) PubChem (443760) PubChem (443761) PubChem (15519421) | |||
| 6 | H (A) | water | water | 18.0 | 441 | Chemie (HOH) |
Sequence modifications
A: 1 - 414 (UniProt: B5L6K6)
| PDB | External Database | Details |
|---|---|---|
| Gly -1 | - | expression tag |
| His 0 | - | expression tag |
| Asn 181 | His 181 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 1 |
| Total formula weight | 46066.9 | |
| Non-Polymers* | Number of molecules | 6 |
| Total formula weight | 1179.4 | |
| All* | Total formula weight | 47246.2 |
