4E2W
X-ray Structure of the H181N mutant of TcaB9, a C-3'-Methyltransferase, in Complex with S-Adenosyl-L-Homocysteine and Sugar Product
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A | TcaB9 | polymer | 416 | 46066.9 | 1 | UniProt (B5L6K6) Pfam (PF08421) Pfam (PF13489) Pfam (PF08484) In PDB | Micromonospora chalcea | C-3'-Methyltransferase |
| 2 | A | ZINC ION | non-polymer | 65.4 | 1 | Chemie (ZN) | |||
| 3 | A | 1,2-ETHANEDIOL | non-polymer | 62.1 | 3 | Chemie (EDO) | |||
| 4 | A | S-ADENOSYL-L-HOMOCYSTEINE | non-polymer | 384.4 | 1 | Chemie (SAH) | |||
| 5 | A | (2R,4S,6R)-4-amino-4,6-dimethyl-5-oxotetrahydro-2H-pyran-2-yl [(2R,3S,5R)-3-hydroxy-5-(5-methyl-2,4-dioxo-3,4-dihydropyrimidin-1(2H)-yl)tetrahydrofuran-2-yl]methyl dihydrogen diphosphate (non-preferred name) | non-polymer | 543.4 | 1 | Chemie (JHZ) | |||
| 6 | water | water | 18.0 | 441 | Chemie (HOH) |
Sequence modifications
A: 1 - 414 (UniProt: B5L6K6)
| PDB | External Database | Details |
|---|---|---|
| Gly -1 | - | expression tag |
| His 0 | - | expression tag |
| Asn 181 | His 181 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 1 |
| Total formula weight | 46066.9 | |
| Non-Polymers* | Number of molecules | 6 |
| Total formula weight | 1179.4 | |
| All* | Total formula weight | 47246.2 |
