4E04

RpBphP2 chromophore-binding domain crystallized by homologue-directed mutagenesis.

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (A, B)	Bacteriophytochrome (Light-regulated signal transduction histidine kinase), PhyB1	polymer	327	36187.1	2	UniProt (Q6N5G3) Pfam (PF08446) Pfam (PF01590)	Rhodopseudomonas palustris
2	C, D (A, B)	3-[2-[(Z)-[3-(2-carboxyethyl)-5-[(Z)-(4-ethenyl-3-methyl-5-oxidanylidene-pyrrol-2-ylidene)methyl]-4-methyl-pyrrol-1-ium -2-ylidene]methyl]-5-[(Z)-[(3E)-3-ethylidene-4-methyl-5-oxidanylidene-pyrrolidin-2-ylidene]methyl]-4-methyl-1H-pyrrol-3- yl]propanoic acid	non-polymer		585.7	2	Chemie (LBV)
3	E, F (A, B)	water	water		18.0	515	Chemie (HOH)

Sequence modifications

A, B: 1 - 319 (UniProt: Q6N5G3)

PDB	External Database	Details
Pro 98	Arg 98	engineered mutation
Asp 99	Lys 99	engineered mutation
Gly 100	Asp 100	engineered mutation
Glu 101	Ala 101	engineered mutation
Arg 102	Gly 102	engineered mutation
Ala 103	Phe 103	engineered mutation
Phe 104	Val 104	engineered mutation
Asn 105	-	insertion
Arg 127	Ala 126	engineered mutation
Tyr 128	Glu 127	engineered mutation
Ser 135	Arg 134	engineered mutation
Val 136	Thr 135	engineered mutation
Arg 137	Asn 136	engineered mutation
Arg 237	Val 236	engineered mutation
Glu 296	Asp 295	engineered mutation
Val 297	Gly 296	engineered mutation
Leu 320	-	expression tag
Glu 321	-	expression tag
His 322	-	expression tag
His 323	-	expression tag
His 324	-	expression tag
His 325	-	expression tag
His 326	-	expression tag
His 327	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	72374.2
Non-Polymers*	Number of molecules	2
	Total formula weight	1171.3
All*	Total formula weight	73545.6

*Water molecules are not included.