4DUV
E. coli (lacZ) beta-galactosidase (G974A) 2-deoxy-galactosyl-enzyme and bis-Tris complex
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, B, C, D | Beta-galactosidase | polymer | 1052 | 119757.8 | 4 | UniProt (P00722) Pfam (PF02837) Pfam (PF00703) Pfam (PF02836) Pfam (PF16353) Pfam (PF02929) In PDB | Escherichia coli | Beta-gal, Lactase |
| 2 | A, B, C, D | 2-deoxy-alpha-D-galactopyranose | non-polymer | 164.2 | 4 | Chemie (2DG) | |||
| 3 | A, B, C, D | 2-[BIS-(2-HYDROXY-ETHYL)-AMINO]-2-HYDROXYMETHYL-PROPANE-1,3-DIOL | non-polymer | 209.2 | 4 | Chemie (BTB) | |||
| 4 | A, B, C, D | MAGNESIUM ION | non-polymer | 24.3 | 9 | Chemie (MG) | |||
| 5 | D, A, B, C | SODIUM ION | non-polymer | 23.0 | 16 | Chemie (NA) | |||
| 6 | A, B, C, D | DIMETHYL SULFOXIDE | non-polymer | 78.1 | 90 | Chemie (DMS) | |||
| 7 | water | water | 18.0 | 4018 | Chemie (HOH) |
Sequence modifications
A, B, C, D: 9 - 1023 (UniProt: P00722)
| PDB | External Database | Details |
|---|---|---|
| Met -28 | - | initiating methionine |
| Gly -27 | - | expression tag |
| Gly -26 | - | expression tag |
| Ser -25 | - | expression tag |
| His -24 | - | expression tag |
| His -23 | - | expression tag |
| His -22 | - | expression tag |
| His -21 | - | expression tag |
| His -20 | - | expression tag |
| His -19 | - | expression tag |
| Gly -18 | - | expression tag |
| Met -17 | - | expression tag |
| Ala -16 | - | expression tag |
| Ser -15 | - | expression tag |
| Met -14 | - | expression tag |
| Thr -13 | - | expression tag |
| Gly -12 | - | expression tag |
| Gly -11 | - | expression tag |
| Gln -10 | - | expression tag |
| Gln -9 | - | expression tag |
| Met -8 | - | expression tag |
| Gly -7 | - | expression tag |
| Arg -6 | - | expression tag |
| Asp -5 | - | expression tag |
| Leu -4 | - | expression tag |
| Tyr -3 | - | expression tag |
| Asp -2 | - | expression tag |
| Asp -1 | - | expression tag |
| Asp 0 | - | expression tag |
| Asp 1 | - | expression tag |
| Lys 2 | - | expression tag |
| Asp 3 | - | expression tag |
| Pro 4 | - | expression tag |
| Met 5 | - | expression tag |
| Ile 6 | - | expression tag |
| Asp 7 | - | expression tag |
| Pro 8 | - | expression tag |
| Ala 794 | Gly 795 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 4 |
| Total formula weight | 479031.1 | |
| Non-Polymers* | Number of molecules | 123 |
| Total formula weight | 9112.1 | |
| All* | Total formula weight | 488143.2 |
