4DSK
Crystal structure of fragment DNA polymerase I from Bacillus stearothermophilus with duplex DNA, PPi and Calcium
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (B) | DNA (5'-D(*GP*GP*CP*TP*AP*CP*AP*GP*GP*AP*CP*TP*CP*G)-3') | polymer | 14 | 4305.8 | 1 | |||
| 2 | B (C) | DNA (5'-D(*TP*CP*AP*CP*GP*AP*GP*TP*CP*CP*TP*GP*TP*AP*GP*CP*C)-3') | polymer | 17 | 5163.3 | 1 | |||
| 3 | C (A) | DNA polymerase | polymer | 579 | 66101.8 | 1 | UniProt (D9N168) Pfam (PF22619) Pfam (PF00476) UniProt (by SIFTS) (E1C9K5) | Geobacillus stearothermophilus | |
| 4 | D, F, G, H (B, A) | CALCIUM ION | non-polymer | 40.1 | 4 | Chemie (CA) | |||
| 5 | E (A) | PYROPHOSPHATE 2- | non-polymer | 176.0 | 1 | Chemie (POP) | |||
| 6 | I, J, K (B, C, A) | water | water | 18.0 | 300 | Chemie (HOH) |
Sequence modifications
A: 298 - 876 (UniProt: D9N168)
| PDB | External Database | Details |
|---|---|---|
| Asp 598 | Ala 301 | engineered mutation |
| Val 713 | Pro 416 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 3 |
| Total formula weight | 75570.9 | |
| Non-Polymers* | Number of molecules | 5 |
| Total formula weight | 336.3 | |
| All* | Total formula weight | 75907.2 |
