4DSF
Ternary complex of Bacillus DNA Polymerase I Large Fragment F710Y, DNA duplex, and rCTP (paired with dG of template) in presence of Mn2+
Entity
Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
1 | A, D | DNA polymerase | polymer | 592 | 67490.3 | 2 | UniProt (Q5KWC1) Pfam (PF00476) In PDB | Geobacillus kaustophilus | |
2 | B, E | DNA (5'-D(*CP*CP*TP*GP*AP*CP*TP*CP*(DOC))-3') | polymer | 9 | 2635.8 | 2 | |||
3 | C, F | DNA (5'-D(*CP*AP*TP*GP*GP*GP*AP*GP*TP*CP*AP*GP*G)-3') | polymer | 13 | 4056.6 | 2 | |||
4 | D | CYTIDINE-5'-TRIPHOSPHATE | non-polymer | 483.2 | 1 | Chemie (CTP) | |||
5 | D | SULFATE ION | non-polymer | 96.1 | 4 | Chemie (SO4) | |||
6 | water | water | 18.0 | 1168 | Chemie (HOH) |
Sequence modifications
A, D: 285 - 876 (UniProt: Q5KWC1)
PDB | External Database | Details |
---|---|---|
Ala 598 | Asp 600 | engineered mutation |
Tyr 710 | Phe 712 | engineered mutation |
His 823 | Arg 825 | SEE REMARK 999 |
Sequence viewer
Contents of the asymmetric unit
Polymers | Number of chains | 6 |
Total formula weight | 148365.4 | |
Non-Polymers* | Number of molecules | 5 |
Total formula weight | 867.4 | |
All* | Total formula weight | 149232.8 |