4DNR

Crystal structure of the CusBA heavy-metal efflux complex from Escherichia coli, E716F mutant

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	B, C	Cation efflux system protein CusB	polymer	413	45179.6	2	UniProt (P77239) Pfam (PF00529) Pfam (PF19335) Pfam (PF16572) In PDB	Escherichia coli
2	A	Cation efflux system protein CusA	polymer	1054	115794.9	1	UniProt (P38054) Pfam (PF00873) In PDB	Escherichia coli
3	A	COPPER (II) ION	non-polymer		63.5	1	Chemie (CU)
4		water	water		18.0	5	Chemie (HOH)

Sequence modifications

B, C: 1 - 407 (UniProt: P77239)

PDB	External Database	Details
His 408	-	expression tag
His 409	-	expression tag
His 410	-	expression tag
His 411	-	expression tag
His 412	-	expression tag
His 413	-	expression tag

A: 1 - 1047 (UniProt: P38054)

PDB	External Database	Details
Met -6	-	expression tag
His -5	-	expression tag
His -4	-	expression tag
His -3	-	expression tag
His -2	-	expression tag
His -1	-	expression tag
His 0	-	expression tag
Phe 716	Glu 716	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	3
	Total formula weight	206154.1
Non-Polymers*	Number of molecules	1
	Total formula weight	63.5
All*	Total formula weight	206217.7

*Water molecules are not included.