4DNF

Crystal structure of the CFTR inhibitory factor Cif with the E153Q mutation adducted with the epibromohydrin hydrolysis intermediate

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C, D (A, B, C, D)	Putative hydrolase	polymer	301	34163.7	4	UniProt (Q02P97) Pfam (PF12697) UniProt (by SIFTS) (A0A0H2ZD27)	Pseudomonas aeruginosa
2	E, F, G, H (A, B, C, D)	(2S)-1-bromopropan-2-ol	non-polymer		139.0	4	Chemie (EBH)
3	I, J, K, L (A, B, C, D)	water	water		18.0	829	Chemie (HOH)

Sequence modifications

A, B, C, D: 25 - 319 (UniProt: Q02P97)

PDB	External Database	Details
Gln 153	Glu 153	engineered mutation
His 320	-	expression tag
His 321	-	expression tag
His 322	-	expression tag
His 323	-	expression tag
His 324	-	expression tag
His 325	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	136654.9
Non-Polymers*	Number of molecules	4
	Total formula weight	556.0
All*	Total formula weight	137210.8

*Water molecules are not included.