4DNE

Crystal structure of a triple-mutant of streptavidin in complex with desthiobiotin

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (A, B)	Streptavidin	polymer	183	18891.8	2	UniProt (P22629) Pfam (PF01382)	Streptomyces avidinii
2	C, F (A, B)	6-(5-METHYL-2-OXO-IMIDAZOLIDIN-4-YL)-HEXANOIC ACID	non-polymer		214.3	2	Chemie (DTB)
3	D, E, G, H, I... (A, B)	SULFATE ION	non-polymer		96.1	6	Chemie (SO4)
4	K, L (A, B)	water	water		18.0	91	Chemie (HOH)

Sequence modifications

A, B: -23 - 159 (UniProt: P22629)

PDB	External Database	Details
Val 44	Glu 68	engineered mutation
Thr 45	Ser 69	engineered mutation
Arg 47	Val 71	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	37783.6
Non-Polymers*	Number of molecules	8
	Total formula weight	1004.9
All*	Total formula weight	38788.5

*Water molecules are not included.