4DJ4
X-ray structure of mutant N211D of bifunctional nuclease TBN1 from Solanum lycopersicum (Tomato)
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, B (A, B) | Nuclease | polymer | 277 | 31647.5 | 2 | UniProt (Q0KFV0) Pfam (PF02265) | Solanum lycopersicum (tomato) | |
| 2 | C (C) | 2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose | branched | 424.4 | 1 | In PDB GlyTouCan (G42666HT) | |||
| 3 | D, F (D, F) | alpha-D-mannopyranose-(1-3)-[alpha-D-mannopyranose-(1-6)]beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose | branched | 910.8 | 2 | In PDB GlyTouCan (G22768VO) | |||
| 4 | E (E) | alpha-D-mannopyranose-(1-3)-beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose | branched | 748.7 | 1 | In PDB GlyTouCan (G81315DD) | |||
| 5 | G, H, I, K, L... (A, B) | ZINC ION | non-polymer | 65.4 | 6 | Chemie (ZN) | |||
| 6 | J, N (A, B) | CHLORIDE ION | non-polymer | 35.5 | 2 | Chemie (CL) | |||
| 7 | O (B) | SODIUM ION | non-polymer | 23.0 | 1 | Chemie (NA) | |||
| 8 | P, Q (A, B) | water | water | 18.0 | 169 | Chemie (HOH) |
Sequence modifications
A, B: 26 - 302 (UniProt: Q0KFV0)
| PDB | External Database | Details |
|---|---|---|
| Asp 211 | Asn 211 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 2 |
| Total formula weight | 63295.0 | |
| Branched | Number of molecules | 4 |
| Total formula weight | 2994.7 | |
| Non-Polymers* | Number of molecules | 9 |
| Total formula weight | 486.4 | |
| All* | Total formula weight | 66776.1 |
