4DI9
CRYSTAL STRUCTURE OF THE D248A mutant of 2-PYRONE-4,6-DICARBOXYLIC ACID HYDROLASE FROM SPHINGOMONAS PAUCIMOBILIS complexed with substrate at pH 6.5
Replaces: 4D9AEntity
Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
1 | A | 2-pyrone-4,6-dicarbaxylate hydrolase | polymer | 303 | 33986.8 | 1 | UniProt (O87170) Pfam (PF04909) In PDB | Sphingomonas paucimobilis | 2-pyrone-4,6-dicarboxylic acid hydrolase |
2 | A | (1E,3Z)-4-hydroxybuta-1,3-diene-1,2,4-tricarboxylic acid | non-polymer | 202.1 | 1 | Chemie (0GY) | |||
3 | A | ACETATE ION | non-polymer | 59.0 | 1 | Chemie (ACT) | |||
4 | water | water | 18.0 | 430 | Chemie (HOH) |
Sequence modifications
A: 4 - 295 (UniProt: O87170)
PDB | External Database | Details |
---|---|---|
Met 1 | - | expression tag |
Ser 2 | - | expression tag |
Leu 3 | - | expression tag |
Ala 248 | Asp 246 | engineered mutation |
Glu 296 | - | expression tag |
Gly 297 | - | expression tag |
His 298 | - | expression tag |
His 299 | - | expression tag |
His 300 | - | expression tag |
His 301 | - | expression tag |
His 302 | - | expression tag |
His 303 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
Polymers | Number of chains | 1 |
Total formula weight | 33986.8 | |
Non-Polymers* | Number of molecules | 2 |
Total formula weight | 261.2 | |
All* | Total formula weight | 34247.9 |