4DG3

Crystal structure of R336A mutant of cAMP-dependent protein kinase with unphosphorylated turn motif.

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (E)	cAMP-dependent protein kinase catalytic subunit alpha	polymer	371	42873.7	1	UniProt (P05132) Pfam (PF00069)	Mus musculus (mouse)	PKA C-alpha
2	B (A)	cAMP-dependent protein kinase inhibitor alpha	polymer	20	2226.4	1	UniProt (P63248)	Mus musculus	PKI-alpha, cAMP-dependent protein kinase inhibitor, muscle/brain isoform
3	C (E)	PHOSPHOAMINOPHOSPHONIC ACID-ADENYLATE ESTER	non-polymer		506.2	1	Chemie (ANP)
4	D, E (E)	MAGNESIUM ION	non-polymer		24.3	2	Chemie (MG)
5	F, G (E, A)	water	water		18.0	249	Chemie (HOH)

Sequence modifications

E: 0 - 350 (UniProt: P05132)

PDB	External Database	Details
Met -20	-	expression tag
Gly -19	-	expression tag
Ser -18	-	expression tag
Ser -17	-	expression tag
His -16	-	expression tag
His -15	-	expression tag
His -14	-	expression tag
His -13	-	expression tag
His -12	-	expression tag
His -11	-	expression tag
Ser -10	-	expression tag
Ser -9	-	expression tag
Gly -8	-	expression tag
Leu -7	-	expression tag
Val -6	-	expression tag
Pro -5	-	expression tag
Arg -4	-	expression tag
Gly -3	-	expression tag
Ser -2	-	expression tag
His -1	-	expression tag
Ala 336	Arg 337	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	45100.2
Non-Polymers*	Number of molecules	3
	Total formula weight	554.8
All*	Total formula weight	45655.0

*Water molecules are not included.