4DG3

Crystal structure of R336A mutant of cAMP-dependent protein kinase with unphosphorylated turn motif.

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	E	cAMP-dependent protein kinase catalytic subunit alpha	polymer	371	42873.7	1	UniProt (P05132) Pfam (PF00069) In PDB	Mus musculus (mouse)	PKA C-alpha
2	A	cAMP-dependent protein kinase inhibitor alpha	polymer	20	2226.4	1	UniProt (P63248) In PDB	Mus musculus	PKI-alpha, cAMP-dependent protein kinase inhibitor, muscle/brain isoform
3	E	PHOSPHOAMINOPHOSPHONIC ACID-ADENYLATE ESTER	non-polymer		506.2	1	Chemie (ANP)
4	E	MAGNESIUM ION	non-polymer		24.3	2	Chemie (MG)
5		water	water		18.0	249	Chemie (HOH)

Sequence modifications

E: 0 - 350 (UniProt: P05132)

PDB	External Database	Details
Met -20	-	EXPRESSION TAG
Gly -19	-	EXPRESSION TAG
Ser -18	-	EXPRESSION TAG
Ser -17	-	EXPRESSION TAG
His -16	-	EXPRESSION TAG
His -15	-	EXPRESSION TAG
His -14	-	EXPRESSION TAG
His -13	-	EXPRESSION TAG
His -12	-	EXPRESSION TAG
His -11	-	EXPRESSION TAG
Ser -10	-	EXPRESSION TAG
Ser -9	-	EXPRESSION TAG
Gly -8	-	EXPRESSION TAG
Leu -7	-	EXPRESSION TAG
Val -6	-	EXPRESSION TAG
Pro -5	-	EXPRESSION TAG
Arg -4	-	EXPRESSION TAG
Gly -3	-	EXPRESSION TAG
Ser -2	-	EXPRESSION TAG
His -1	-	EXPRESSION TAG
Ala 336	Arg 337	ENGINEERED MUTATION

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	45100.2
Non-Polymers*	Number of molecules	3
	Total formula weight	554.8
All*	Total formula weight	45655.0

*Water molecules are not included.