4DFY

Crystal structure of R194A mutant of cAMP-dependent protein kinase with unphosphorylated activation loop

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (A, E)	cAMP-dependent protein kinase catalytic subunit alpha	polymer	371	42793.8	2	UniProt (P05132) Pfam (PF00069)	Mus musculus (mouse)	PKA C-alpha
2	C, D (A, E)	water	water		18.0	7	Chemie (HOH)

Sequence modifications

A, E: 0 - 350 (UniProt: P05132)

PDB	External Database	Details
Met -20	-	expression tag
Gly -19	-	expression tag
Ser -18	-	expression tag
Ser -17	-	expression tag
His -16	-	expression tag
His -15	-	expression tag
His -14	-	expression tag
His -13	-	expression tag
His -12	-	expression tag
His -11	-	expression tag
Ser -10	-	expression tag
Ser -9	-	expression tag
Gly -8	-	expression tag
Leu -7	-	expression tag
Val -6	-	expression tag
Pro -5	-	expression tag
Arg -4	-	expression tag
Gly -3	-	expression tag
Ser -2	-	expression tag
His -1	-	expression tag
Ala 194	Arg 195	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	85587.5
All*	Total formula weight	85587.5

*Water molecules are not included.