4DF7
Crystal structure of Staphylococcal nuclease variant Delta+PHS V23L/V99I at cryogenic temperature
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (A) | Thermonuclease | polymer | 143 | 16171.5 | 1 | UniProt (P00644) Pfam (PF00565) | Staphylococcus aureus | TNase, Micrococcal nuclease, Staphylococcal nuclease |
| 2 | B (A) | CALCIUM ION | non-polymer | 40.1 | 1 | Chemie (CA) | |||
| 3 | C (A) | THYMIDINE-3',5'-DIPHOSPHATE | non-polymer | 402.2 | 1 | Chemie (THP) | |||
| 4 | D (A) | water | water | 18.0 | 95 | Chemie (HOH) |
Sequence modifications
A: 1 - 149 (UniProt: P00644)
| PDB | External Database | Details |
|---|---|---|
| Leu 23 | Val 105 | engineered mutation |
| - | Thr 126 | deletion |
| - | Lys 127 | deletion |
| - | His 128 | deletion |
| - | Pro 129 | deletion |
| - | Lys 130 | deletion |
| - | Lys 131 | deletion |
| Phe 50 | Gly 132 | engineered mutation |
| Asn 51 | Val 133 | engineered mutation |
| Ile 99 | Val 181 | engineered mutation |
| Gly 117 | Pro 199 | engineered mutation |
| Leu 124 | His 206 | engineered mutation |
| Ala 128 | Ser 210 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 1 |
| Total formula weight | 16171.5 | |
| Non-Polymers* | Number of molecules | 2 |
| Total formula weight | 442.3 | |
| All* | Total formula weight | 16613.8 |
