4DCM

Crystal Structure of methyltransferase RlmG modifying G1835 of 23S rRNA in Escherichia coli

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	Ribosomal RNA large subunit methyltransferase G	polymer	375	42460.4	1	UniProt (P42596) Pfam (PF05175)	Escherichia coli	23S rRNA m2G1835 methyltransferase, rRNA (guanine-N(2)-)-methyltransferase RlmG
2	B (A)	S-ADENOSYLMETHIONINE	non-polymer		398.4	1	Chemie (SAM)
3	C, D (A)	DI(HYDROXYETHYL)ETHER	non-polymer		106.1	2	Chemie (PEG)
4	E (A)	water	water		18.0	128	Chemie (HOH)

Sequence modifications

A: 9 - 373 (UniProt: P42596)

PDB	External Database	Details
His 7	-	expression tag
Mse 8	-	expression tag
Leu 374	-	expression tag
Glu 375	-	expression tag
His 376	-	expression tag
His 377	-	expression tag
His 378	-	expression tag
His 379	-	expression tag
His 380	-	expression tag
His 381	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	1
	Total formula weight	42460.4
Non-Polymers*	Number of molecules	3
	Total formula weight	610.7
All*	Total formula weight	43071.1

*Water molecules are not included.