4DAJ

Structure of the M3 Muscarinic Acetylcholine Receptor

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C, D (A, B, C, D)	Muscarinic acetylcholine receptor M3, Lysozyme	polymer	479	54990.3	4	UniProt (P08483) UniProt (P00720) Pfam (PF00001) Pfam (PF00959)	Rattus norvegicus (brown rat rat rats)	Endolysin, Lysis protein, Muramidase
2	E, J, L, O (A, B, C, D)	(1R,2R,4S,5S,7S)-7-{[hydroxy(dithiophen-2-yl)acetyl]oxy}-9,9-dimethyl-3-oxa-9-azoniatricyclo[3.3.1.0~2,4~]nonane	non-polymer		392.5	4	Chemie (0HK) PubChem (60831) PubChem (131951) PubChem (50909376) PubChem (123134303) PubChem (58667495) PubChem (59084585) PubChem (59168855) PubChem (59288009) PubChem (60003260) PubChem (87909275) PubChem (98047845) PubChem (122130800) PubChem (124527228) PubChem (656611) PubChem (3086655) PubChem (5487427) PubChem (5748851) PubChem (9804844) PubChem (11598033) PubChem (15057856)
3	F, G, H, I, K... (A, B, C, D)	PHOSPHATE ION	non-polymer		95.0	10	Chemie (PO4) PubChem (1061)

Sequence modifications

A, B, C, D: 57 - 259 (UniProt: P08483)

PDB	External Database	Details
Gly 56	-	expression tag

A, B, C, D: 1001 - 1161 (UniProt: P00720)

PDB	External Database	Details
Thr 1054	Cys 54	engineered mutation
Ala 1097	Cys 97	engineered mutation

A, B, C, D: 482 - 589 (UniProt: P08483)

PDB	External Database	Details
His 590	-	expression tag
His 591	-	expression tag
His 592	-	expression tag
His 593	-	expression tag
His 594	-	expression tag
His 595	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	219961.2
Non-Polymers*	Number of molecules	14
	Total formula weight	2519.8
All*	Total formula weight	222480.9

*Water molecules are not included.