4DAE

Crystal structure of the hexameric purine nucleoside phosphorylase from Bacillus subtilis in complex with 6-chloroguanosine

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	Purine nucleoside phosphorylase deoD-type	polymer	253	27562.1	1	UniProt (O34925) Pfam (PF01048)	Bacillus subtilis	PNP, Purine nucleoside phosphorylase II, PU-NPase II
2	B (A)	6-chloro-9-(beta-D-ribofuranosyl)-9H-purin-2-amine	non-polymer		301.7	1	Chemie (6CR)
3	C (A)	CHLORIDE ION	non-polymer		35.5	1	Chemie (CL)
4	D (A)	ACETATE ION	non-polymer		59.0	1	Chemie (ACT)
5	E, F (A)	GLYCEROL	non-polymer		92.1	2	Chemie (GOL)
6	G (A)	water	water		18.0	67	Chemie (HOH)

Sequence modifications

A: 1 - 233 (UniProt: O34925)

PDB	External Database	Details
Met -19	-	expression tag
Gly -18	-	expression tag
Ser -17	-	expression tag
Ser -16	-	expression tag
His -15	-	expression tag
His -14	-	expression tag
His -13	-	expression tag
His -12	-	expression tag
His -11	-	expression tag
His -10	-	expression tag
Ser -9	-	expression tag
Ser -8	-	expression tag
Gly -7	-	expression tag
Leu -6	-	expression tag
Val -5	-	expression tag
Pro -4	-	expression tag
Arg -3	-	expression tag
Gly -2	-	expression tag
Ser -1	-	expression tag
His 0	-	expression tag
Asp 225	Glu 225	SEE REMARK 999

Contents of the asymmetric unit

Polymers	Number of chains	1
	Total formula weight	27562.1
Non-Polymers*	Number of molecules	5
	Total formula weight	580.4
All*	Total formula weight	28142.5

*Water molecules are not included.