4DA1

Crystal structure of branched-chain alpha-ketoacid dehydrogenase phosphatase with Mg (II) ions at the active site

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	Protein phosphatase 1K, mitochondrial	polymer	389	43338.8	1	UniProt (Q8N3J5) Pfam (PF00481)	Homo sapiens (human)	PP2C domain-containing protein phosphatase 1K, PP2C-like mitochondrial protein, PP2C-type mitochondrial phosphoprotein phosphatase, PTMP, Protein phosphatase 2C isoform kappa, PP2C-kappa
2	B, C, D (A)	BETA-MERCAPTOETHANOL	non-polymer		78.1	3	Chemie (BME)
3	E, F, G (A)	MAGNESIUM ION	non-polymer		24.3	3	Chemie (MG)
4	H (A)	water	water		18.0	64	Chemie (HOH)

Sequence modifications

A: 84 - 360 (UniProt: Q8N3J5)

PDB	External Database	Details
Gly -28	-	expression tag
His -27	-	expression tag
His -26	-	expression tag
His -25	-	expression tag
His -24	-	expression tag
His -23	-	expression tag
His -22	-	expression tag
Gly -21	-	expression tag
Ser -20	-	expression tag
Asp -19	-	expression tag
Ser -18	-	expression tag
Glu -17	-	expression tag
Val -16	-	expression tag
Asn -15	-	expression tag
Gln -14	-	expression tag
Glu -13	-	expression tag
Ala -12	-	expression tag
Lys -11	-	expression tag
Pro -10	-	expression tag
Glu -9	-	expression tag
Val -8	-	expression tag
Lys -7	-	expression tag
Pro -6	-	expression tag
Glu -5	-	expression tag
Val -4	-	expression tag
Lys -3	-	expression tag
Pro -2	-	expression tag
Glu -1	-	expression tag
Thr 0	-	expression tag
His 1	-	expression tag
Ile 2	-	expression tag
Asn 3	-	expression tag
Leu 4	-	expression tag
Lys 5	-	expression tag
Val 6	-	expression tag
Ser 7	-	expression tag
Asp 8	-	expression tag
Gly 9	-	expression tag
Ser 10	-	expression tag
Ser 11	-	expression tag
Glu 12	-	expression tag
Ile 13	-	expression tag
Phe 14	-	expression tag
Phe 15	-	expression tag
Lys 16	-	expression tag
Ile 17	-	expression tag
Lys 18	-	expression tag
Lys 19	-	expression tag
Thr 20	-	expression tag
Thr 21	-	expression tag
Pro 22	-	expression tag
Leu 23	-	expression tag
Arg 24	-	expression tag
Arg 25	-	expression tag
Leu 26	-	expression tag
Met 27	-	expression tag
Glu 28	-	expression tag
Ala 29	-	expression tag
Phe 30	-	expression tag
Ala 31	-	expression tag
Lys 32	-	expression tag
Arg 33	-	expression tag
Gln 34	-	expression tag
Gly 35	-	expression tag
Lys 36	-	expression tag
Glu 37	-	expression tag
Met 38	-	expression tag
Asp 39	-	expression tag
Ser 40	-	expression tag
Leu 41	-	expression tag
Arg 42	-	expression tag
Phe 43	-	expression tag
Leu 44	-	expression tag
Tyr 45	-	expression tag
Asp 46	-	expression tag
Gly 47	-	expression tag
Ile 48	-	expression tag
Arg 49	-	expression tag
Ile 50	-	expression tag
Gln 51	-	expression tag
Ala 52	-	expression tag
Asp 53	-	expression tag
Gln 54	-	expression tag
Ala 55	-	expression tag
Pro 56	-	expression tag
Glu 57	-	expression tag
Asp 58	-	expression tag
Leu 59	-	expression tag
Asp 60	-	expression tag
Met 61	-	expression tag
Glu 62	-	expression tag
Asp 63	-	expression tag
Asn 64	-	expression tag
Asp 65	-	expression tag
Ile 66	-	expression tag
Ile 67	-	expression tag
Glu 68	-	expression tag
Ala 69	-	expression tag
His 70	-	expression tag
Arg 71	-	expression tag
Glu 72	-	expression tag
Gln 73	-	expression tag
Ile 74	-	expression tag
Gly 75	-	expression tag
Gly 76	-	expression tag
Glu 77	-	expression tag
Asn 78	-	expression tag
Leu 79	-	expression tag
Tyr 80	-	expression tag
Phe 81	-	expression tag
Gln 82	-	expression tag
Ala 83	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	1
	Total formula weight	43338.8
Non-Polymers*	Number of molecules	6
	Total formula weight	307.3
All*	Total formula weight	43646.1

*Water molecules are not included.