4D72
Crystal structure of a family 98 glycoside hydrolase catalytic module (Sp3GH98) in complex with the type 2 blood group A-tetrasaccharide (E558A L19 mutant)
Entity
Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
1 | A | GLYCOSIDE HYDROLASE | polymer | 596 | 68681.2 | 1 | UniProt (A5LBQ0) Pfam (PF08306) Pfam (PF08307) UniProt (by SIFTS) (A0A0H2UKY4) In PDB | STREPTOCOCCUS PNEUMONIAE | |
2 | B | alpha-L-fucopyranose-(1-2)-beta-D-galactopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose | branched | 529.5 | 1 | In PDB BIRD (PRD_900036) GlyTouCan (G61459JW) | H type 2 antigen, beta anomer | ||
3 | A | 2-acetamido-2-deoxy-alpha-D-galactopyranose | non-polymer | 221.2 | 1 | Chemie (A2G) | |||
4 | A | 1,2-ETHANEDIOL | non-polymer | 62.1 | 2 | Chemie (EDO) | |||
5 | water | water | 18.0 | 565 | Chemie (HOH) |
Sequence modifications
A: 421 - 1003 (UniProt: A5LBQ0)
PDB | External Database | Details |
---|---|---|
Leu 1004 | - | expression tag |
Glu 1005 | - | expression tag |
His 1006 | - | expression tag |
Pro 1007 | - | expression tag |
Pro 1008 | - | expression tag |
Pro 1009 | - | expression tag |
Pro 1010 | - | expression tag |
Pro 1011 | - | expression tag |
Leu 1012 | - | expression tag |
Arg 1013 | - | expression tag |
Ser 1014 | - | expression tag |
Gly 1015 | - | expression tag |
Cys 1016 | - | expression tag |
His 530 | Tyr 532 | engineered mutation |
Ala 558 | Glu 560 | engineered mutation |
Ser 559 | Asn 561 | engineered mutation |
Ser 592 | Asn 594 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
Polymers | Number of chains | 1 |
Total formula weight | 68681.2 | |
Branched | Number of molecules | 1 |
Total formula weight | 529.5 | |
Non-Polymers* | Number of molecules | 3 |
Total formula weight | 345.3 | |
All* | Total formula weight | 69556.0 |