4CXK

G9 mutant of PAS, arylsulfatase from Pseudomonas Aeruginosa

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B	ARYLSULFATASE	polymer	536	59919.3	2	UniProt (P51691) Pfam (PF00884) In PDB	PSEUDOMONAS AERUGINOSA	AS, ARYL-SULFATE SULPHOHYDROLASE
2	A, B	CALCIUM ION	non-polymer		40.1	2	Chemie (CA)
3	A, B	SULFATE ION	non-polymer		96.1	2	Chemie (SO4)
4	A, B	DI(HYDROXYETHYL)ETHER	non-polymer		106.1	2	Chemie (PEG)
5		water	water		18.0	973	Chemie (HOH)

Sequence modifications

A, B: 1 - 534 (UniProt: P51691)

PDB	External Database	Details
Glu 535	-	expression tag
Arg 536	-	expression tag
Thr 48	Ala 48	engineered mutation
Ala 50	Thr 50	engineered mutation
Leu 69	Ile 69	engineered mutation
Val 72	Met 72	engineered mutation
Asp 337	Gly 337	engineered mutation
Ser 352	Arg 352	engineered mutation
Gly 461	Glu 461	engineered mutation
Asp 523	Glu 523	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	119838.6
Non-Polymers*	Number of molecules	6
	Total formula weight	484.5
All*	Total formula weight	120323.1

*Water molecules are not included.