4CWM

High-glycosylation crystal structure of the bifunctional endonuclease (AtBFN2) from Arabidopsis thaliana

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (A, B)	ENDONUCLEASE 2	polymer	269	30606.2	2	UniProt (Q9C9G4) Pfam (PF02265)	ARABIDOPSIS THALIANA (THALE CRESS)	ATENDO2, DEOXYRIBONUCLEASE ENDO2, SINGLE-STRANDED-NUCLEATE ENDONUCLEASE ENDO2, BIFUNCTIONAL ENDONUCLEASE ATBFN2
2	C, F (C, F)	alpha-D-mannopyranose-(1-3)-[alpha-D-mannopyranose-(1-6)]beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose	branched		910.8	2	In PDB GlyTouCan (G22768VO)
3	D, E (D, E)	2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose	branched		424.4	2	In PDB GlyTouCan (G42666HT)
4	G, H (G, H)	beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose	branched		586.5	2	In PDB GlyTouCan (G15407YE)
5	I, J, K, L, M... (A, B)	ZINC ION	non-polymer		65.4	6	Chemie (ZN)
6	O, P (A, B)	water	water		18.0	457	Chemie (HOH)

Sequence modifications

A, B: 1 - 263 (UniProt: Q9C9G4)

PDB	External Database	Details
His 264	-	expression tag
His 265	-	expression tag
His 266	-	expression tag
His 267	-	expression tag
His 268	-	expression tag
His 269	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	61212.3
Branched	Number of molecules	6
	Total formula weight	3843.5
Non-Polymers*	Number of molecules	6
	Total formula weight	392.5
All*	Total formula weight	65448.3

*Water molecules are not included.