4CTH

Neprilysin variant G399V,G714K in complex with phosphoramidon

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	NEPRILYSIN	polymer	709	81414.5	1	UniProt (P08473) Pfam (PF05649) Pfam (PF01431) In PDB	HOMO SAPIENS (HUMAN)	ATRIOPEPTIDASE, COMMON ACUTE LYMPHOCYTIC LEUKEMIA ANTIGEN, CALLA, ENKEPHALINASE, NEUTRAL ENDOPEPTIDASE 24.11, NEP, NEUTRAL ENDOPEPTIDASE, SKIN FIBROBLAST ELASTASE, SFE, CD10
2	A	2-acetamido-2-deoxy-beta-D-glucopyranose	non-polymer		221.2	3	Chemie (NAG)
3	A	ZINC ION	non-polymer		65.4	1	Chemie (ZN)
4	A	GLYCEROL	non-polymer		92.1	8	Chemie (GOL)
5	A	N-ALPHA-L-RHAMNOPYRANOSYLOXY(HYDROXYPHOSPHINYL)-L-LEUCYL-L-TRYPTOPHAN	non-polymer		543.5	1	BIRD (PRD_000638) Chemie (RDF)
6		water	water		18.0	325	Chemie (HOH)

Sequence modifications

A: 51 - 749 (UniProt: P08473)

PDB	External Database	Details
His 41	-	expression tag
His 42	-	expression tag
His 43	-	expression tag
His 44	-	expression tag
His 45	-	expression tag
His 46	-	expression tag
His 47	-	expression tag
His 48	-	expression tag
His 49	-	expression tag
His 50	-	expression tag
Val 399	Gly 400	engineered mutation
Lys 714	Gly 715	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	1
	Total formula weight	81414.5
Non-Polymers*	Number of molecules	13
	Total formula weight	2009.3
All*	Total formula weight	83423.8

*Water molecules are not included.