4CR3
Deep classification of a large cryo-EM dataset defines the conformational landscape of the 26S proteasome
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (1) | PROTEASOME COMPONENT PRE3 | polymer | 215 | 23573.6 | 1 | UniProt (P38624) Pfam (PF00227) | SACCHAROMYCES CEREVISIAE (BAKER'S YEAST) | 20S PROTEASOME BETA SUBUNIT 1, MACROPAIN SUBUNIT PRE3, MULTICATALYTIC ENDOPEPTIDASE COMPLEX SUBUNIT PRE3, PROTEINASE YSCE SUBUNIT PRE3 |
| 2 | B (2) | PROTEASOME COMPONENT PUP1 | polymer | 261 | 28299.9 | 1 | UniProt (P25043) Pfam (PF00227) Pfam (PF12465) | SACCHAROMYCES CEREVISIAE (BAKER'S YEAST) | 20S PROTEASOME BETA SUBUNIT 2 MACROPAIN SUBUNIT PUP1, MULTICATALYTIC ENDOPEPTIDASE COMPLEX SUBUNIT PUP1, PROTEINASE YSCE SUBUNIT PUP1 |
| 3 | C (3) | PROTEASOME COMPONENT PUP3 | polymer | 205 | 22627.8 | 1 | UniProt (P25451) Pfam (PF00227) | SACCHAROMYCES CEREVISIAE (BAKER'S YEAST) | 20S PROTEASOME BETA SUBUNIT 3, MACROPAIN SUBUNIT PUP3, MULTICATALYTIC ENDOPEPTIDASE COMPLEX SUBUNIT PUP3 |
| 4 | D (4) | PROTEASOME COMPONENT C11 | polymer | 198 | 22545.7 | 1 | UniProt (P22141) Pfam (PF00227) | SACCHAROMYCES CEREVISIAE (BAKER'S YEAST) | 20S PROTEASOME BETA SUBUNIT 4, MACROPAIN SUBUNIT C11, MULTICATALYTIC ENDOPEPTIDASE COMPLEX SUBUNIT C11, PROTEINASE YSCE SUBUNIT 11 |
| 5 | E (5) | PROTEASOME COMPONENT PRE2 | polymer | 287 | 31698.6 | 1 | UniProt (P30656) Pfam (PF00227) | SACCHAROMYCES CEREVISIAE (BAKER'S YEAST) | 20S PROTEASOME BETA SUBUNIT 5, MACROPAIN SUBUNIT PRE2, MULTICATALYTIC ENDOPEPTIDASE COMPLEX SUBUNIT PRE2, PROTEINASE YSCE SUBUNIT PRE2 |
| 6 | F (6) | PROTEASOME COMPONENT C5 | polymer | 241 | 26905.1 | 1 | UniProt (P23724) Pfam (PF00227) | SACCHAROMYCES CEREVISIAE (BAKER'S YEAST) | 20S PROTEASOME BETA SUBUNIT 6, MULTICATALYTIC ENDOPEPTIDASE |
| 7 | G (7) | PROTEASOME COMPONENT PRE4 | polymer | 266 | 29471.3 | 1 | UniProt (P30657) Pfam (PF00227) | SACCHAROMYCES CEREVISIAE (BAKER'S YEAST) | 20S PROTEASOME BETA SUBUNIT 7, MACROPAIN SUBUNIT PRE4, MULTICATALYTIC ENDOPEPTIDASE COMPLEX SUBUNIT PRE4, PROTEINASE YSCE SUBUNIT PRE4 |
| 8 | H (A) | PROTEASOME COMPONENT C7-ALPHA | polymer | 252 | 28033.8 | 1 | UniProt (P21243) Pfam (PF00227) Pfam (PF10584) | SACCHAROMYCES CEREVISIAE (BAKER'S YEAST) | 20S PROTEASOME ALPHA SUBUNIT 1, MACROPAIN SUBUNIT C7-ALPHA, MULTICATALYTIC ENDOPEPTIDASE COMPLEX C7, PROTEASOME COMPONENT Y8, PROTEINASE YSCE SUBUNIT 7, SCL1 SUPPRESSOR PROTEIN |
| 9 | I (B) | PROTEASOME COMPONENT Y7 | polymer | 250 | 27191.8 | 1 | UniProt (P23639) Pfam (PF10584) Pfam (PF00227) | SACCHAROMYCES CEREVISIAE (BAKER'S YEAST) | 20S PROTEASOME ALPHA SUBUNIT 2, MACROPAIN SUBUNIT Y7, MULTICATALYTIC ENDOPEPTIDASE COMPLEX SUBUNIT Y7, PROTEINASE YSCE SUBUNIT 7 |
| 10 | J (C) | PROTEASOME COMPONENT Y13 | polymer | 258 | 28748.2 | 1 | UniProt (P23638) Pfam (PF10584) Pfam (PF00227) | SACCHAROMYCES CEREVISIAE (BAKER'S YEAST) | 20S PROTEASOME ALPHA SUBUNIT 3, MACROPAIN SUBUNIT Y13, MULTICATALYTIC ENDOPEPTIDASE COMPLEX SUBUNIT Y13, PROTEINASE YSCE SUBUNIT 13 |
| 11 | K (D) | PROTEASOME COMPONENT PRE6 | polymer | 254 | 28478.1 | 1 | UniProt (P40303) Pfam (PF10584) Pfam (PF00227) | SACCHAROMYCES CEREVISIAE (BAKER'S YEAST) | 20S PROTEASOME ALPHA SUBUNIT 4, MACROPAIN SUBUNIT PRE6, MULTICATALYTIC ENDOPEPTIDASE COMPLEX SUBUNIT PRE6, PROTEINASE YSCE SUBUNIT PRE6 |
| 12 | L (E) | PROTEASOME COMPONENT PUP2 | polymer | 260 | 28649.1 | 1 | UniProt (P32379) Pfam (PF10584) Pfam (PF00227) | SACCHAROMYCES CEREVISIAE (BAKER'S YEAST) | 20S PROTEASOME ALPHA SUBUNIT 5, MACROPAIN SUBUNIT PUP2, MULTICATALYTIC ENDOPEPTIDASE COMPLEX SUBUNIT PUP2, PROTEINASE YSCE SUBUNIT PUP2 |
| 13 | M (F) | PROTEASOME COMPONENT PRE5 | polymer | 234 | 25634.0 | 1 | UniProt (P40302) Pfam (PF10584) Pfam (PF00227) | SACCHAROMYCES CEREVISIAE (BAKER'S YEAST) | 20S PROTEASOME ALPHA SUBUNIT 6, MACROPAIN SUBUNIT PRE5, MULTICATALYTIC ENDOPEPTIDASE COMPLEX SUBUNIT PRE5, PROTEINASE YSCE SUBUNIT PRE5 |
| 14 | N (G) | PROTEASOME COMPONENT C1 | polymer | 288 | 31575.1 | 1 | UniProt (P21242) Pfam (PF10584) Pfam (PF00227) | SACCHAROMYCES CEREVISIAE (BAKER'S YEAST) | 20S PROTEASOME ALPHA SUBUNIT 7, MACROPAIN SUBUNIT C1, MULTICATALYTIC ENDOPEPTIDASE COMPLEX SUBUNIT C1, PROTEINASE YSCE SUBUNIT 1 |
| 15 | O (H) | 26S PROTEASE REGULATORY SUBUNIT 7 HOMOLOG | polymer | 467 | 52054.9 | 1 | UniProt (P33299) Pfam (PF21236) Pfam (PF00004) Pfam (PF17862) | SACCHAROMYCES CEREVISIAE (BAKER'S YEAST) | RPT1, PROTEIN CIM5, TAT-BINDING HOMOLOG 3 |
| 16 | P (I) | 26S PROTEASE REGULATORY SUBUNIT 4 HOMOLOG | polymer | 437 | 48898.2 | 1 | UniProt (P40327) Pfam (PF16450) Pfam (PF00004) Pfam (PF17862) | SACCHAROMYCES CEREVISIAE (BAKER'S YEAST) | RPT2, TAT-BINDING HOMOLOG 5 |
| 17 | Q (J) | 26S PROTEASE REGULATORY SUBUNIT 8 HOMOLOG | polymer | 405 | 45342.7 | 1 | UniProt (Q01939) Pfam (PF16450) Pfam (PF00004) Pfam (PF17862) | SACCHAROMYCES CEREVISIAE (BAKER'S YEAST) | RPT6, PROTEIN CIM3, PROTEIN SUG1, TAT-BINDING PROTEIN TBY1 |
| 18 | R (K) | 26S PROTEASE REGULATORY SUBUNIT 6B HOMOLOG | polymer | 428 | 47953.7 | 1 | UniProt (P33298) Pfam (PF16450) Pfam (PF00004) | SACCHAROMYCES CEREVISIAE (BAKER'S YEAST) | RPT3, PROTEIN YNT1, TAT-BINDING HOMOLOG 2 |
| 19 | S (L) | 26S PROTEASE SUBUNIT RPT4 | polymer | 437 | 49480.1 | 1 | UniProt (P53549) Pfam (PF16450) Pfam (PF00004) Pfam (PF17862) | SACCHAROMYCES CEREVISIAE (BAKER'S YEAST) | RPT4,26S PROTEASE SUBUNIT SUG2, PROTEASOMAL CAP SUBUNIT |
| 20 | T (M) | 26S PROTEASE REGULATORY SUBUNIT 6A | polymer | 434 | 48315.7 | 1 | UniProt (P33297) Pfam (PF16450) Pfam (PF00004) Pfam (PF17862) | SACCHAROMYCES CEREVISIAE (BAKER'S YEAST) | RPT5, TAT-BINDING PROTEIN HOMOLOG 1, TBP-1 |
| 21 | U (N) | 26S PROTEASOME REGULATORY SUBUNIT RPN2 | polymer | 945 | 104351.9 | 1 | UniProt (P32565) Pfam (PF21505) Pfam (PF01851) Pfam (PF13646) Pfam (PF18004) | SACCHAROMYCES CEREVISIAE (BAKER'S YEAST) | RPN2 |
| 22 | V (O) | 26S PROTEASOME REGULATORY SUBUNIT RPN9 | polymer | 393 | 45839.3 | 1 | UniProt (Q04062) Pfam (PF22037) Pfam (PF01399) Pfam (PF18261) | SACCHAROMYCES CEREVISIAE (BAKER'S YEAST) | RPN9, PROTEASOME NON-ATPASE SUBUNIT 7 |
| 23 | W (P) | 26S PROTEASOME REGULATORY SUBUNIT RPN5 | polymer | 445 | 51840.4 | 1 | UniProt (Q12250) Pfam (PF22241) Pfam (PF01399) Pfam (PF18098) | SACCHAROMYCES CEREVISIAE (BAKER'S YEAST) | RPN5, PROTEASOME NON-ATPASE SUBUNIT 5 |
| 24 | X (Q) | 26S PROTEASOME REGULATORY SUBUNIT RPN6 | polymer | 434 | 49839.8 | 1 | UniProt (Q12377) Pfam (PF18055) Pfam (PF01399) Pfam (PF18503) | SACCHAROMYCES CEREVISIAE (BAKER'S YEAST) | RPN6, PROTEASOME NON-ATPASE SUBUNIT 4 |
| 25 | Y (R) | 26S PROTEASOME REGULATORY SUBUNIT RPN7 | polymer | 429 | 49016.4 | 1 | UniProt (Q06103) Pfam (PF10602) Pfam (PF01399) Pfam (PF21154) | SACCHAROMYCES CEREVISIAE (BAKER'S YEAST) | RPN7 |
| 26 | Z (S) | 26S PROTEASOME REGULATORY SUBUNIT RPN3 | polymer | 523 | 60464.6 | 1 | UniProt (P40016) Pfam (PF01399) Pfam (PF08375) | SACCHAROMYCES CEREVISIAE (BAKER'S YEAST) | RPN3 |
| 27 | AA (T) | 26S PROTEASOME REGULATORY SUBUNIT RPN12 | polymer | 274 | 31952.1 | 1 | UniProt (P32496) Pfam (PF10075) | SACCHAROMYCES CEREVISIAE (BAKER'S YEAST) | RPN12, NUCLEAR INTEGRITY PROTEIN 1 |
| 28 | BA (U) | 26S PROTEASOME REGULATORY SUBUNIT RPN8 | polymer | 338 | 38365.5 | 1 | UniProt (Q08723) Pfam (PF01398) Pfam (PF13012) | SACCHAROMYCES CEREVISIAE (BAKER'S YEAST) | RPN8 |
| 29 | CA (V) | 26S PROTEASOME REGULATORY SUBUNIT RPN11 | polymer | 306 | 34442.3 | 1 | UniProt (P43588) Pfam (PF01398) | SACCHAROMYCES CEREVISIAE (BAKER'S YEAST) | RPN11, PROTEIN MPR1 |
| 30 | DA (W) | 26S PROTEASOME REGULATORY SUBUNIT RPN10 | polymer | 268 | 29776.1 | 1 | UniProt (P38886) Pfam (PF13519) | SACCHAROMYCES CEREVISIAE (BAKER'S YEAST) | RPN10 |
| 31 | EA (X) | 26S PROTEASOME REGULATORY SUBUNIT RPN13 | polymer | 156 | 17919.0 | 1 | UniProt (O13563) Pfam (PF04683) | SACCHAROMYCES CEREVISIAE (BAKER'S YEAST) | RPN13, PROTEASOME NON-ATPASE SUBUNIT 13 |
| 32 | FA (Y) | 26S PROTEASOME COMPLEX SUBUNIT SEM1 | polymer | 89 | 10397.1 | 1 | UniProt (O94742) Pfam (PF05160) | SACCHAROMYCES CEREVISIAE (BAKER'S YEAST) | |
| 33 | GA (Z) | 26S PROTEASOME REGULATORY SUBUNIT RPN1 | polymer | 993 | 109601.9 | 1 | UniProt (P38764) Pfam (PF17781) Pfam (PF01851) Pfam (PF18051) | SACCHAROMYCES CEREVISIAE (BAKER'S YEAST) | HMG-COA REDUCTASE DEGRADATION PROTEIN 2, PROTEASOME NON-ATPASE SUBUNIT 1 |
Sequence modifications
5: -74 - 212 (UniProt: P30656)
| PDB | External Database | Details |
|---|---|---|
| Arg 33 | Lys 108 | SEE REMARK 999 |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 33 |
| Total formula weight | 1309283.8 | |
| All* | Total formula weight | 1309283.8 |
