4CNX

Surface residue engineering of bovine carbonic anhydrase to an extreme halophilic enzyme for potential application in postcombustion CO2 capture

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	CARBONIC ANHYDRASE 2	polymer	262	29426.5	1	UniProt (P00921) Pfam (PF00194) In PDB	BOS TAURUS (CATTLE)	CARBONATE DEHYDRATASE II, CARBONIC ANHYDRASE II, CA-II
2	A	ZINC ION	non-polymer		65.4	1	Chemie (ZN)
3	A	DI(HYDROXYETHYL)ETHER	non-polymer		106.1	1	Chemie (PEG)
4		water	water		18.0	405	Chemie (HOH)

Sequence modifications

A: 1 - 260 (UniProt: P00921)

PDB	External Database	Details
Met -1	-	expression tag
Gly 0	-	expression tag
Asp 8	Gly 8	engineered mutation
Glu 18	Lys 18	engineered mutation
Asp 24	Asn 24	engineered mutation
Asp 36	Lys 36	engineered mutation
Asp 39	Val 39	engineered mutation
Asp 50	Val 50	engineered mutation
Asp 57	Arg 57	engineered mutation
Asp 62	Asn 62	engineered mutation
Glu 74	Gln 74	engineered mutation
Asp 85	Thr 85	engineered mutation
Glu 136	Gln 136	engineered mutation
Glu 169	Lys 169	engineered mutation
Asp 177	Asn 177	engineered mutation
Glu 186	Asn 186	engineered mutation
Glu 220	Gln 220	engineered mutation
Glu 238	Leu 238	engineered mutation
Asp 252	Asn 252	engineered mutation
Glu 254	Gln 254	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	1
	Total formula weight	29426.5
Non-Polymers*	Number of molecules	2
	Total formula weight	171.5
All*	Total formula weight	29598.0

*Water molecules are not included.