4CNW
Surface residue engineering of bovine carbonic anhydrase to an extreme halophilic enzyme for potential application in postcombustion CO2 capture
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, B (A, B) | CARBONIC ANHYDRASE 2 | polymer | 262 | 29394.6 | 2 | UniProt (P00921) Pfam (PF00194) | BOS TAURUS (CATTLE) | CARBONATE DEHYDRATASE II, CARBONIC ANHYDRASE II, CA-II |
| 2 | C, D (A, B) | ZINC ION | non-polymer | 65.4 | 2 | Chemie (ZN) | |||
| 3 | E (B) | CALCIUM ION | non-polymer | 40.1 | 1 | Chemie (CA) | |||
| 4 | F, G (A, B) | water | water | 18.0 | 281 | Chemie (HOH) |
Sequence modifications
A, B: 1 - 260 (UniProt: P00921)
| PDB | External Database | Details |
|---|---|---|
| Met -1 | - | expression tag |
| Gly 0 | - | expression tag |
| Asp 8 | Gly 8 | engineered mutation |
| Asp 24 | Asn 24 | engineered mutation |
| Asp 36 | Lys 36 | engineered mutation |
| Asp 39 | Val 39 | engineered mutation |
| Asp 50 | Val 50 | engineered mutation |
| Asp 57 | Arg 57 | engineered mutation |
| Asp 62 | Asn 62 | engineered mutation |
| Glu 74 | Gln 74 | engineered mutation |
| Glu 136 | Gln 136 | engineered mutation |
| Glu 169 | Lys 169 | engineered mutation |
| Glu 238 | Leu 238 | engineered mutation |
| Asp 252 | Asn 252 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 2 |
| Total formula weight | 58789.1 | |
| Non-Polymers* | Number of molecules | 3 |
| Total formula weight | 170.9 | |
| All* | Total formula weight | 58960.0 |
