4CNG

Crystal structure of Sulfolobus acidocaldarius TrmJ in complex with S-adenosyl-L-Homocysteine

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (A, B)	SPOU RRNA METHYLASE	polymer	255	29080.9	2	UniProt (Q4JB16) Pfam (PF00588)	SULFOLOBUS ACIDOCALDARIUS	TRNA METHYLTRANSFERASE, TRMJ
2	C, D, F (A, B)	S-ADENOSYL-L-HOMOCYSTEINE	non-polymer		384.4	3	Chemie (SAH)
3	E (B)	GLYCEROL	non-polymer		92.1	1	Chemie (GOL)
4	G, H (A, B)	water	water		18.0	89	Chemie (HOH)

Sequence modifications

A, B: 1 - 235 (UniProt: Q4JB16)

PDB	External Database	Details
Met -19	-	expression tag
Gly -18	-	expression tag
Ser -17	-	expression tag
Ser -16	-	expression tag
His -15	-	expression tag
His -14	-	expression tag
His -13	-	expression tag
His -12	-	expression tag
His -11	-	expression tag
His -10	-	expression tag
Ser -9	-	expression tag
Ser -8	-	expression tag
Gly -7	-	expression tag
Leu -6	-	expression tag
Val -5	-	expression tag
Pro -4	-	expression tag
Arg -3	-	expression tag
Gly -2	-	expression tag
Ser -1	-	expression tag
His 0	-	expression tag
Ala 38	Cys 38	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	58161.8
Non-Polymers*	Number of molecules	4
	Total formula weight	1245.3
All*	Total formula weight	59407.2

*Water molecules are not included.