4CNE
Crystal structure of E.coli TrmJ in complex with S-adenosyl-L- homocysteine
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, B (A, B) | TRNA (CYTIDINE/URIDINE-2'-O-)-METHYLTRANSFERASE TRMJ | polymer | 267 | 29420.5 | 2 | UniProt (P0AE01) Pfam (PF00588) | ESCHERICHIA COLI | TRNA (CYTIDINE(32)/URIDINE(32)-2'-O)-METHYLTRANSFERASE, TRNA CM32/UM32 METHYLTRANSFERASE, TRMJ |
| 2 | C, D (A, B) | S-ADENOSYL-L-HOMOCYSTEINE | non-polymer | 384.4 | 2 | Chemie (SAH) PubChem (16760024) PubChem (193) PubChem (13792) PubChem (177678424) PubChem (189149) PubChem (439155) PubChem (25210495) PubChem (21125603) PubChem (44361184) PubChem (25203782) PubChem (46936206) PubChem (50989525) PubChem (51380914) PubChem (52224398) PubChem (57544532) PubChem (52224400) PubChem (59046843) PubChem (59170628) PubChem (59576228) PubChem (66805708) PubChem (5284331) PubChem (70689219) PubChem (70702054) PubChem (70832431) PubChem (71170554) PubChem (13231890) PubChem (6419189) PubChem (118374891) PubChem (118560068) PubChem (126969526) PubChem (129105892) PubChem (164157219) PubChem (143518505) PubChem (154578335) PubChem (163054214) PubChem (163335525) | |||
| 3 | E (B) | DI(HYDROXYETHYL)ETHER | non-polymer | 106.1 | 1 | Chemie (PEG) PubChem (8117) | |||
| 4 | F, G (A, B) | water | water | 18.0 | 43 | Chemie (HOH) |
Sequence modifications
A, B: 1 - 246 (UniProt: P0AE01)
| PDB | External Database | Details |
|---|---|---|
| Met -16 | - | expression tag |
| Arg -15 | - | expression tag |
| Gly -14 | - | expression tag |
| Ser -13 | - | expression tag |
| His -12 | - | expression tag |
| His -11 | - | expression tag |
| His -10 | - | expression tag |
| His -9 | - | expression tag |
| His -8 | - | expression tag |
| His -7 | - | expression tag |
| Thr -6 | - | expression tag |
| Asp -5 | - | expression tag |
| Pro -4 | - | expression tag |
| Ala -3 | - | expression tag |
| Leu -2 | - | expression tag |
| Arg -1 | - | expression tag |
| Ala 1 | Met 1 | engineered mutation |
| Gly 247 | - | expression tag |
| Leu 248 | - | expression tag |
| Cys 249 | - | expression tag |
| Gly 250 | - | expression tag |
| Arg 251 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 2 |
| Total formula weight | 58841.0 | |
| Non-Polymers* | Number of molecules | 3 |
| Total formula weight | 874.9 | |
| All* | Total formula weight | 59716.0 |
