4CND

Crystal structure of E.coli TrmJ

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (A, B)	TRNA (CYTIDINE/URIDINE-2'-O-)-METHYLTRANSFERASE TRMJ	polymer	267	29420.5	2	UniProt (P0AE01) Pfam (PF00588)	ESCHERICHIA COLI	TRNA (CYTIDINE(32)/URIDINE(32)-2'-O)-METHYLTRANSFERASE, TRNA CM32/UM32 METHYLTRANSFERASE, TRMJ
2	C (B)	DI(HYDROXYETHYL)ETHER	non-polymer		106.1	1	Chemie (PEG)
3	D, E (A, B)	water	water		18.0	207	Chemie (HOH)

Sequence modifications

A, B: 1 - 246 (UniProt: P0AE01)

PDB	External Database	Details
Met -16	-	expression tag
Arg -15	-	expression tag
Gly -14	-	expression tag
Ser -13	-	expression tag
His -12	-	expression tag
His -11	-	expression tag
His -10	-	expression tag
His -9	-	expression tag
His -8	-	expression tag
His -7	-	expression tag
Thr -6	-	expression tag
Asp -5	-	expression tag
Pro -4	-	expression tag
Ala -3	-	expression tag
Leu -2	-	expression tag
Arg -1	-	expression tag
Ala 1	Met 1	engineered mutation
Gly 247	-	expression tag
Leu 248	-	expression tag
Cys 249	-	expression tag
Gly 250	-	expression tag
Arg 251	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	58841.0
Non-Polymers*	Number of molecules	1
	Total formula weight	106.1
All*	Total formula weight	58947.2

*Water molecules are not included.