4CN6

GlgE isoform 1 from Streptomyces coelicolor E423A mutant with maltose bound

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B	ALPHA-1,4-GLUCAN\:MALTOSE-1-PHOSPHATE MALTOSYLTRANSFERASE 1	polymer	695	77506.8	2	UniProt (Q9L1K2) Pfam (PF11896) Pfam (PF21702) In PDB	STREPTOMYCES COELICOLOR	PEP1A, SCO5443, (1->4)-ALPHA-D-GLUCAN\: MALTOSE-1-PHOSPHATE ALPHA-D-MALTOSYLTRANSFERASE 1
2	C, D	alpha-D-glucopyranose-(1-4)-beta-D-glucopyranose	branched		342.3	2	In PDB BIRD (PRD_900018) GlyTouCan (G66120GK)		beta-maltose
3		water	water		18.0	480	Chemie (HOH)

Sequence modifications

A, B: 1 - 675 (UniProt: Q9L1K2)

PDB	External Database	Details
Met -19	-	expression tag
Gly -18	-	expression tag
Ser -17	-	expression tag
Ser -16	-	expression tag
His -15	-	expression tag
His -14	-	expression tag
His -13	-	expression tag
His -12	-	expression tag
His -11	-	expression tag
His -10	-	expression tag
Ser -9	-	expression tag
Ser -8	-	expression tag
Gly -7	-	expression tag
Leu -6	-	expression tag
Val -5	-	expression tag
Pro -4	-	expression tag
Arg -3	-	expression tag
Gly -2	-	expression tag
Ser -1	-	expression tag
His 0	-	expression tag
Ala 423	Glu 423	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	155013.6
Branched	Number of molecules	2
	Total formula weight	684.6
All*	Total formula weight	155698.2

*Water molecules are not included.