4CFQ

Ca-bound truncated (delta13C) and C3S, C81S and C86S mutated S100A4 complexed with non-muscle myosin IIA

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C, D (A, B, C, D)	PROTEIN S100-A4	polymer	91	10369.7	4	UniProt (P26447) Pfam (PF01023)	HOMO SAPIENS (HUMAN)	CALVASCULIN, METASTASIN, PLACENTAL CALCIUM-BINDING PROTEIN, PROTEIN MTS1, S100 CALCIUM-BINDING PROTEIN A4, S100A4
2	E, F (Q, R)	MYOSIN-9	polymer	45	5358.2	2	UniProt (P35579)	HOMO SAPIENS (HUMAN)	CELLULAR MYOSIN HEAVY CHAIN\,TYPE A, MYOSIN HEAVY CHAIN 9, MYOSIN HEAVY CHAIN\,NON-MUSCLE IIA, NON-MUSCLE MYOSIN HEAVY CHAIN A, NMMHC-A, NON-MUSCLE MYOSIN HEAVY CHAIN IIA, NMMHC II-A, NMMHC-IIA
3	G, H, I, J, K... (A, B, C, D)	CALCIUM ION	non-polymer		40.1	8	Chemie (CA)
4	O, P, Q, R, S... (A, B, C, D, Q...)	water	water		18.0	318	Chemie (HOH)

Sequence modifications

A, B, C, D: 1 - 88 (UniProt: P26447)

PDB	External Database	Details
Gly -2	-	expression tag
Ser -1	-	expression tag
His 0	-	expression tag
Ser 3	Cys 3	engineered mutation
Ser 81	Cys 81	engineered mutation
Ser 86	Cys 86	engineered mutation

Q, R: 1893 - 1937 (UniProt: P35579)

PDB	External Database	Details
Tyr 1893	Arg 1893	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	6
	Total formula weight	52195.4
Non-Polymers*	Number of molecules	8
	Total formula weight	320.6
All*	Total formula weight	52516.0

*Water molecules are not included.