4C7P

Crystal structure of Legionella pneumophila RalF F255K mutant

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	RALF	polymer	402	45523.0	1	UniProt (Q8RT31) Pfam (PF01369) Pfam (PF18248)	LEGIONELLA PNEUMOPHILA	GUANINE EXCHANGE FACTOR RALF
2	B (A)	GLYCEROL	non-polymer		92.1	1	Chemie (GOL)

Sequence modifications

A: 2 - 374 (UniProt: Q8RT31)

PDB	External Database	Details
Met -27	-	expression tag
Ser -26	-	expression tag
Tyr -25	-	expression tag
Tyr -24	-	expression tag
His -23	-	expression tag
His -22	-	expression tag
His -21	-	expression tag
His -20	-	expression tag
His -19	-	expression tag
His -18	-	expression tag
Asp -17	-	expression tag
Tyr -16	-	expression tag
Asp -15	-	expression tag
Ile -14	-	expression tag
Pro -13	-	expression tag
Thr -12	-	expression tag
Thr -11	-	expression tag
Glu -10	-	expression tag
Asn -9	-	expression tag
Leu -8	-	expression tag
Tyr -7	-	expression tag
Phe -6	-	expression tag
Gln -5	-	expression tag
Gly -4	-	expression tag
Ala -3	-	expression tag
Met -2	-	expression tag
Gly -1	-	expression tag
Ala 0	-	expression tag
Ser 1	-	expression tag
Lys 255	Phe 255	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	1
	Total formula weight	45523.0
Non-Polymers*	Number of molecules	1
	Total formula weight	92.1
All*	Total formula weight	45615.1

*Water molecules are not included.