4C29

Crystal Structure of High-Affinity von Willebrand Factor A1 domain with Disulfide Mutation

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B	VON WILLEBRAND FACTOR	polymer	215	24706.7	2	UniProt (P04275) Pfam (PF00092) In PDB	HOMO SAPIENS (HUMAN)	VWF, VON WILLEBRAND ANTIGEN 2, VON WILLEBRAND ANTIGEN II
2	A	ACETATE ION	non-polymer		59.0	1	Chemie (ACT)
3	A, B	DI(HYDROXYETHYL)ETHER	non-polymer		106.1	4	Chemie (PEG)
4	A, B	CALCIUM ION	non-polymer		40.1	3	Chemie (CA)
5		water	water		18.0	175	Chemie (HOH)

Sequence modifications

A, B: 1264 - 1471 (UniProt: P04275)

PDB	External Database	Details
Met 1263	-	expression tag
His 1472	-	expression tag
His 1473	-	expression tag
His 1474	-	expression tag
His 1475	-	expression tag
His 1476	-	expression tag
His 1477	-	expression tag
Cys 1271	Tyr 1271	engineered mutation
Arg 1272	Cys 1272	engineered mutation
Ala 1381	Thr 1381	variant

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	49413.4
Non-Polymers*	Number of molecules	8
	Total formula weight	603.8
All*	Total formula weight	50017.1

*Water molecules are not included.