4BWX

Structure of Neurospora crassa PAN3 pseudokinase mutant

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (A, B)	PAB-DEPENDENT POLY(A)-SPECIFIC RIBONUCLEASE SUBUNIT PAN-3	polymer	429	49380.3	2	UniProt (Q7SDP4) Pfam (PF18101)	NEUROSPORA CRASSA	PAB1P-DEPENDENT POLY(A)-NUCLEASE, PAB-DEPENDENT POLYA-SPECIFIC RIBONUCLEASE SUBUNIT PAN-3
2	C, E (A, B)	PHOSPHOTHIOPHOSPHORIC ACID-ADENYLATE ESTER	non-polymer		523.2	2	Chemie (AGS)
3	D, F (A, B)	MAGNESIUM ION	non-polymer		24.3	2	Chemie (MG)

Sequence modifications

A, B: 234 - 656 (UniProt: Q7SDP4)

PDB	External Database	Details
Gly 228	-	expression tag
Pro 229	-	expression tag
His 230	-	expression tag
Met 231	-	expression tag
Leu 232	-	expression tag
Glu 233	-	expression tag
Met 510	Leu 510	engineered mutation
Pro 511	Gly 511	engineered mutation
Met 512	Gly 512	engineered mutation
Gly 514	Thr 514	engineered mutation
Ala 515	Thr 515	engineered mutation
Arg 516	His 516	engineered mutation
Phe 517	Leu 517	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	98760.6
Non-Polymers*	Number of molecules	4
	Total formula weight	1095.1
All*	Total formula weight	99855.7

*Water molecules are not included.