4BWM
KlenTaq mutant in complex with a RNA/DNA hybrid
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (A) | DNA POLYMERASE I, THERMOSTABLE | polymer | 540 | 61057.1 | 1 | UniProt (P19821) Pfam (PF09281) Pfam (PF00476) | THERMUS AQUATICUS | TAQ POLYMERASE 1 |
| 2 | B (B) | 5'-D(*GP*AP*CP*CP*AP*CP*GP*GP*CP*GP*CP*DOCP)-3' | polymer | 12 | 3617.4 | 1 | SYNTHETIC CONSTRUCT | PRIMER | |
| 3 | C (G) | 5'-R(*AP*AP*AP*GP*GP*GP*CP*GP*CP*CP*GP*UP*GP*GP*UP*C)-3' | polymer | 16 | 5192.2 | 1 | SYNTHETIC CONSTRUCT | TEMPLATE | |
| 4 | D (A) | 2',3'-DIDEOXYCYTIDINE 5'-TRIPHOSPHATE | non-polymer | 451.2 | 1 | Chemie (DCT) | |||
| 5 | E, F (A) | CHLORIDE ION | non-polymer | 35.5 | 2 | Chemie (CL) | |||
| 6 | G, K, L (A, B) | MAGNESIUM ION | non-polymer | 24.3 | 3 | Chemie (MG) | |||
| 7 | H, I, J, M (A, G) | FORMIC ACID | non-polymer | 46.0 | 4 | Chemie (FMT) | |||
| 8 | N, O, P (A, B, G) | water | water | 18.0 | 631 | Chemie (HOH) |
Sequence modifications
A: 293 - 832 (UniProt: P19821)
| PDB | External Database | Details |
|---|---|---|
| Met 459 | Leu 459 | engineered mutation |
| Arg 515 | Ser 515 | engineered mutation |
| Phe 638 | Ile 638 | engineered mutation |
| Lys 747 | Met 747 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 3 |
| Total formula weight | 69866.6 | |
| Non-Polymers* | Number of molecules | 10 |
| Total formula weight | 779.1 | |
| All* | Total formula weight | 70645.7 |
