4BWA
PylRS Y306G, Y384F, I405R mutant in complex with adenylated norbornene
Entity
Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
1 | A | PYRROLYSINE--TRNA LIGASE | polymer | 291 | 33338.1 | 1 | UniProt (Q8PWY1) Pfam (PF01409) In PDB | METHANOSARCINA MAZEI | PYRROLYSINE--TRNA(PYL) LIGASE, PYRROLYSYL-TRNA SYNTHETASE, PYLRS |
2 | A | MAGNESIUM ION | non-polymer | 24.3 | 1 | Chemie (MG) | |||
3 | A | Adenylated Norbornene | non-polymer | 625.6 | 1 | Chemie (N0B) | |||
4 | A | DI(HYDROXYETHYL)ETHER | non-polymer | 106.1 | 1 | Chemie (PEG) | |||
5 | A | 1,2-ETHANEDIOL | non-polymer | 62.1 | 2 | Chemie (EDO) | |||
6 | water | water | 18.0 | 33 | Chemie (HOH) |
Sequence modifications
A: 185 - 454 (UniProt: Q8PWY1)
PDB | External Database | Details |
---|---|---|
Met 164 | - | expression tag |
Gly 165 | - | expression tag |
Ser 166 | - | expression tag |
Ser 167 | - | expression tag |
His 168 | - | expression tag |
His 169 | - | expression tag |
His 170 | - | expression tag |
His 171 | - | expression tag |
His 172 | - | expression tag |
His 173 | - | expression tag |
Ser 174 | - | expression tag |
Ser 175 | - | expression tag |
Gly 176 | - | expression tag |
Leu 177 | - | expression tag |
Val 178 | - | expression tag |
Pro 179 | - | expression tag |
Arg 180 | - | expression tag |
Gly 181 | - | expression tag |
Ser 182 | - | expression tag |
His 183 | - | expression tag |
Met 184 | - | expression tag |
Gly 306 | Tyr 306 | engineered mutation |
Phe 384 | Tyr 384 | engineered mutation |
Arg 405 | Ile 405 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
Polymers | Number of chains | 1 |
Total formula weight | 33338.1 | |
Non-Polymers* | Number of molecules | 5 |
Total formula weight | 880.1 | |
All* | Total formula weight | 34218.3 |