4BU1

Crystal structure of Rad4 BRCT1,2 in complex with a Crb2 phosphopeptide

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (A, B)	S-M CHECKPOINT CONTROL PROTEIN RAD4	polymer	186	21236.5	2	UniProt (P32372) Pfam (PF00533) Pfam (PF12738)	SCHIZOSACCHAROMYCES POMBE (FISSION YEAST)	P74, PROTEIN CUT5
2	C, D (C, D)	DNA REPAIR PROTEIN RHP9	polymer	14	1571.6	2	UniProt (P87074)	SCHIZOSACCHAROMYCES POMBE	RAD9 HOMOLOG, CRB2
3	E, J (A, B)	PHOSPHATE ION	non-polymer		95.0	2	Chemie (PO4)
4	F, K (A, B)	GLYCEROL	non-polymer		92.1	2	Chemie (GOL)
5	G, L (A, B)	PENTAETHYLENE GLYCOL	non-polymer		238.3	2	Chemie (1PE)
6	H, I, M, N, O... (A, B, C)	1,2-ETHANEDIOL	non-polymer		62.1	11	Chemie (EDO)
7	V, W, X, Y (A, B, C, D)	water	water		18.0	353	Chemie (HOH)

Sequence modifications

A, B: 1 - 186 (UniProt: P32372)

PDB	External Database	Details
Leu 98	Phe 98	variant

C, D: 229 - 241 (UniProt: P87074)

PDB	External Database	Details
Gly 228	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	45616.2
Non-Polymers*	Number of molecules	17
	Total formula weight	1533.4
All*	Total formula weight	47149.6

*Water molecules are not included.