4BST

Structure of the ectodomain of LGR5 in complex with R-spondin-1 (Fu1Fu2) in P6122 crystal form

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (A, B)	LEUCINE-RICH REPEAT-CONTAINING G-PROTEIN COUPLED RECEPTOR 5	polymer	539	60366.5	2	UniProt (O75473) Pfam (PF01462) Pfam (PF13855) Pfam (PF12799)	HOMO SAPIENS (HUMAN)	LGR5, G-PROTEIN COUPLED RECEPTOR 49, G-PROTEIN COUPLED RECEPTOR 67, G-PROTEIN COUPLED RECEPTOR HG38
2	C, D (C, D)	R-SPONDIN-1	polymer	126	13706.8	2	UniProt (Q2MKA7) Pfam (PF15913)	HOMO SAPIENS (HUMAN)	ROOF PLATE-SPECIFIC SPONDIN-1, HRSPO1
3	E (E)	beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose	branched		586.5	1	In PDB GlyTouCan (G15407YE)
4	F, G, H, I (A, B)	2-acetamido-2-deoxy-beta-D-glucopyranose	non-polymer		221.2	4	Chemie (NAG)

Sequence modifications

A, B: 22 - 543 (UniProt: O75473)

PDB	External Database	Details
His 8	-	expression tag
His 9	-	expression tag
His 10	-	expression tag
His 11	-	expression tag
His 12	-	expression tag
His 13	-	expression tag
Glu 14	-	expression tag
Asn 15	-	expression tag
Leu 16	-	expression tag
Tyr 17	-	expression tag
Phe 18	-	expression tag
Gln 19	-	expression tag
Gly 20	-	expression tag
Ser 21	-	expression tag
Ala 544	-	expression tag
Ala 545	-	expression tag
Ala 546	-	expression tag

C, D: 31 - 146 (UniProt: Q2MKA7)

PDB	External Database	Details
Gly 29	-	expression tag
Ser 30	-	expression tag
Ala 147	-	expression tag
Ala 148	-	expression tag
His 149	-	expression tag
His 150	-	expression tag
His 151	-	expression tag
His 152	-	expression tag
His 153	-	expression tag
His 154	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	148146.5
Branched	Number of molecules	1
	Total formula weight	586.5
Non-Polymers*	Number of molecules	4
	Total formula weight	884.8
All*	Total formula weight	149617.8

*Water molecules are not included.