4BPZ

Crystal structure of lamA_E269S from Zobellia galactanivorans in complex with a trisaccharide of 1,3-1,4-beta-D-glucan.

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (A, B)	ENDO-1,3-BETA-GLUCANASE, FAMILY GH16	polymer	256	29219.9	2	UniProt (G0L5X4) Pfam (PF00722)	ZOBELLIA GALACTANIVORANS
2	C, D (C, D)	beta-D-glucopyranose-(1-4)-beta-D-glucopyranose-(1-3)-alpha-D-glucopyranose	branched		504.4	2	In PDB GlyTouCan (G89925KE)
3	E, F (A, B)	CALCIUM ION	non-polymer		40.1	2	Chemie (CA)
4	G, H (A, B)	water	water		18.0	584	Chemie (HOH)

Sequence modifications

A, B: 136 - 383 (UniProt: G0L5X4)

PDB	External Database	Details
His 128	-	expression tag
His 129	-	expression tag
His 130	-	expression tag
His 131	-	expression tag
His 132	-	expression tag
His 133	-	expression tag
Gly 134	-	expression tag
Ser 135	-	expression tag
Ser 269	Glu 269	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	58439.8
Branched	Number of molecules	2
	Total formula weight	1008.9
Non-Polymers*	Number of molecules	2
	Total formula weight	80.2
All*	Total formula weight	59528.8

*Water molecules are not included.