4BLU

Crystal structure of Escherichia coli 23S rRNA (A2030-N6)- methyltransferase RlmJ

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (A, B)	RIBOSOMAL RNA LARGE SUBUNIT METHYLTRANSFERASE J	polymer	289	33088.9	2	UniProt (P37634) Pfam (PF04378)	ESCHERICHIA COLI
2	C, K (A, B)	2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL	non-polymer		122.1	2	Chemie (TRS)
3	D, L (A, B)	GLYCEROL	non-polymer		92.1	2	Chemie (GOL)
4	E, F, G, H, I... (A, B)	1,2-ETHANEDIOL	non-polymer		62.1	14	Chemie (EDO)
5	J, Y (A, B)	SULFATE ION	non-polymer		96.1	2	Chemie (SO4)
6	M, N, O (B)	DI(HYDROXYETHYL)ETHER	non-polymer		106.1	3	Chemie (PEG)
7	AA, Z (B, A)	water	water		18.0	557	Chemie (HOH)

Sequence modifications

A, B: 1 - 280 (UniProt: P37634)

PDB	External Database	Details
Ser 281	-	expression tag
Lys 282	-	expression tag
Gly 283	-	expression tag
His 284	-	expression tag
His 285	-	expression tag
His 286	-	expression tag
His 287	-	expression tag
His 288	-	expression tag
His 289	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	66177.7
Non-Polymers*	Number of molecules	23
	Total formula weight	1807.9
All*	Total formula weight	67985.7

*Water molecules are not included.