4BLB
Crystal structure of a human Suppressor of fused (SUFU)-GLI1p complex
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, B, C, D (A, B, C, D) | MALTOSE-BINDING PERIPLASMIC PROTEIN, SUPPRESSOR OF FUSED HOMOLOG | polymer | 753 | 83634.1 | 4 | UniProt (P0AEX9) UniProt (Q9UMX1) Pfam (PF01547) Pfam (PF05076) Pfam (PF12470) | ESCHERICHIA COLI | SUFUH, LINKER, SUPPRESOR OF FUSED |
| 2 | E, F, G, H (E, F, G, H) | ZINC FINGER PROTEIN GLI1 | polymer | 17 | 1649.8 | 4 | UniProt (P08151) | HOMO SAPIENS | TRANSCRIPTIONAL ACTIVATOR GL1, GLIOMA-ASSOCIATED ONCOGENE, ONCOGENE GLI |
| 3 | I, J, K, L (I, J, K, L) | alpha-D-glucopyranose-(1-4)-alpha-D-glucopyranose | branched | 342.3 | 4 | In PDB BIRD (PRD_900001) GlyTouCan (G07411ON) | alpha-maltose | ||
| 4 | M, N, O, P (A, B, C, D) | ZINC ION | non-polymer | 65.4 | 4 | Chemie (ZN) |
Sequence modifications
A, B, C, D: 2 - 368 (UniProt: P0AEX9)
A, B, C, D: 372 - 618 (UniProt: Q9UMX1)
A, B, C, D: 626 - 718 (UniProt: Q9UMX1)
A, B, C, D: 719 - 745 (UniProt: Q9UMX1)
| PDB | External Database | Details |
|---|---|---|
| Met 1 | - | expression tag |
| Thr 3 | Ile 28 | engineered mutation |
| Ala 360 | Glu 385 | engineered mutation |
| Ala 363 | Lys 388 | engineered mutation |
| Ala 364 | Asp 389 | engineered mutation |
| Asn 368 | Arg 393 | engineered mutation |
| Ala 369 | - | linker |
| Ala 370 | - | linker |
| Ala 371 | - | linker |
| His 216 | Ala 241 | engineered mutation |
| His 220 | Lys 245 | engineered mutation |
| - | Glu 454 | deletion |
| - | Glu 455 | deletion |
| - | Phe 456 | deletion |
| Val 746 | - | expression tag |
| Glu 747 | - | expression tag |
| His 748 | - | expression tag |
| His 749 | - | expression tag |
| His 750 | - | expression tag |
| His 751 | - | expression tag |
| His 752 | - | expression tag |
| His 753 | - | expression tag |
| PDB | External Database | Details |
|---|---|---|
| Asp 401 | Trp 61 | engineered mutation |
| Ser 402 | Leu 62 | engineered mutation |
| Phe 403 | Gly 63 | engineered mutation |
| PDB | External Database | Details |
|---|---|---|
| Pro 619 | - | linker |
| Ser 620 | - | linker |
| Arg 621 | - | linker |
| Gly 622 | - | linker |
| Pro 625 | - | linker |
| Ala 718 | Pro 453 | engineered mutation |
| PDB | External Database | Details |
|---|---|---|
| Ala 719 | Lys 457 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 8 |
| Total formula weight | 341135.8 | |
| Branched | Number of molecules | 4 |
| Total formula weight | 1369.2 | |
| Non-Polymers* | Number of molecules | 4 |
| Total formula weight | 261.6 | |
| All* | Total formula weight | 342766.6 |
