4BK2
Crystal structure of 3-hydroxybenzoate 6-hydroxylase uncovers lipid- assisted flavoprotein strategy for regioselective aromatic hydroxylation: Q301E mutant
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (A) | PROBABLE SALICYLATE MONOOXYGENASE | polymer | 424 | 46949.2 | 1 | UniProt (Q0SFK6) Pfam (PF01494) | RHODOCOCCUS JOSTII | 3-HYDROXYBENZOATE 6-HYDROXYLASE |
| 2 | B (A) | FLAVIN-ADENINE DINUCLEOTIDE | non-polymer | 785.5 | 1 | Chemie (FAD) | |||
| 3 | C (A) | PHOSPHATIDYLGLYCEROL-PHOSPHOGLYCEROL | non-polymer | 873.0 | 1 | Chemie (P3A) | |||
| 4 | D (A) | water | water | 18.0 | 81 | Chemie (HOH) |
Sequence modifications
A: 1 - 399 (UniProt: Q0SFK6)
| PDB | External Database | Details |
|---|---|---|
| Lys 400 | - | expression tag |
| Leu 401 | - | expression tag |
| Gly 402 | - | expression tag |
| Pro 403 | - | expression tag |
| Glu 404 | - | expression tag |
| Gln 405 | - | expression tag |
| Lys 406 | - | expression tag |
| Leu 407 | - | expression tag |
| Ile 408 | - | expression tag |
| Ser 409 | - | expression tag |
| Glu 410 | - | expression tag |
| Glu 411 | - | expression tag |
| Asp 412 | - | expression tag |
| Leu 413 | - | expression tag |
| Asn 414 | - | expression tag |
| Ser 415 | - | expression tag |
| Ala 416 | - | expression tag |
| Val 417 | - | expression tag |
| Asp 418 | - | expression tag |
| His 419 | - | expression tag |
| His 420 | - | expression tag |
| His 421 | - | expression tag |
| His 422 | - | expression tag |
| His 423 | - | expression tag |
| His 424 | - | expression tag |
| Glu 301 | Gln 301 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 1 |
| Total formula weight | 46949.2 | |
| Non-Polymers* | Number of molecules | 2 |
| Total formula weight | 1658.5 | |
| All* | Total formula weight | 48607.8 |
