4BJR
Crystal structure of the complex between Prokaryotic Ubiquitin-like Protein Pup and its Ligase PafA
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, B (A, B) | PUP--PROTEIN LIGASE, PROKARYOTIC UBIQUITIN-LIKE PROTEIN PUP | polymer | 517 | 57505.8 | 2 | UniProt (Q8NQE1) UniProt (Q8NQE0) Pfam (PF03136) Pfam (PF05639) | CORYNEBACTERIUM GLUTAMICUM | PROTEASOME ACCESSORY FACTOR A, PUP-CONJUGATING ENZYME, BACTERIAL UBIQUITIN-LIKE MODIFIER |
| 2 | C, E (A, B) | ADENOSINE-5'-TRIPHOSPHATE | non-polymer | 507.2 | 2 | Chemie (ATP) | |||
| 3 | D, F, G (A, B) | MAGNESIUM ION | non-polymer | 24.3 | 3 | Chemie (MG) | |||
| 4 | H, I (A, B) | water | water | 18.0 | 134 | Chemie (HOH) |
Sequence modifications
A, B: 2 - 482 (UniProt: Q8NQE1)
A, B: 489 - 515 (UniProt: Q8NQE0)
| PDB | External Database | Details |
|---|---|---|
| Gly -1 | - | expression tag |
| Leu 0 | - | expression tag |
| Lys 1 | - | expression tag |
| Asn 64 | Asp 64 | engineered mutation |
| Gly 483 | - | linker |
| Ser 484 | - | linker |
| Ala 485 | - | linker |
| Ser 486 | - | linker |
| Gly 487 | - | linker |
| Thr 488 | - | linker |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 2 |
| Total formula weight | 115011.7 | |
| Non-Polymers* | Number of molecules | 5 |
| Total formula weight | 1087.3 | |
| All* | Total formula weight | 116098.9 |
