4BJR

Crystal structure of the complex between Prokaryotic Ubiquitin-like Protein Pup and its Ligase PafA

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B	PUP--PROTEIN LIGASE, PROKARYOTIC UBIQUITIN-LIKE PROTEIN PUP	polymer	517	57505.8	2	UniProt (Q8NQE1) UniProt (Q8NQE0) Pfam (PF03136) Pfam (PF05639) In PDB	CORYNEBACTERIUM GLUTAMICUM	PROTEASOME ACCESSORY FACTOR A, PUP-CONJUGATING ENZYME, BACTERIAL UBIQUITIN-LIKE MODIFIER
2	A, B	ADENOSINE-5'-TRIPHOSPHATE	non-polymer		507.2	2	Chemie (ATP)
3	A, B	MAGNESIUM ION	non-polymer		24.3	3	Chemie (MG)
4		water	water		18.0	134	Chemie (HOH)

Sequence modifications

A, B: 2 - 482 (UniProt: Q8NQE1)

PDB	External Database	Details
Gly -1	-	expression tag
Leu 0	-	expression tag
Lys 1	-	expression tag
Asn 64	Asp 64	engineered mutation
Gly 483	-	linker
Ser 484	-	linker
Ala 485	-	linker
Ser 486	-	linker
Gly 487	-	linker
Thr 488	-	linker

A, B: 489 - 515 (UniProt: Q8NQE0)

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	115011.7
Non-Polymers*	Number of molecules	5
	Total formula weight	1087.3
All*	Total formula weight	116098.9

*Water molecules are not included.